Immune complex size and complement regulate cytokine production by peripheral blood mononuclear cells
Immune complex size and complement regulate cytokine production by peripheral blood mononuclear cells
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DOI：
10.1006/clim.1999.4792
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发表时间：
1999-12-01
影响因子：
8.6
通讯作者：
del Rio, AQ
中科院分区：
文献类型：
--
作者：
Jarvis, JN;Xu, CS;del Rio, AQ
我们以前已经表明，从幼年类风湿性关节炎患儿分离的免疫复合物在大小、组成和促炎能力上是异质的。这里描述的实验仅仅是为了进一步阐明大小和组成在决定免疫复合物的促炎作用中的作用。我们孵育外周血单核细胞（PBMC）与不同的可溶性免疫复合物制剂：调理复合物，这是在血清的存在下形成的，unopsonized复合物，这是在没有血清的情况下形成的，和免疫沉淀溶解后，其形成的补体。ELISA测定显示，在补体存在下形成的免疫复合物在诱导白细胞分泌LL-1 β和IL-8方面的效率低于未调理的复合物。溶解的免疫沉淀物表现出中等的能力，刺激释放两种细胞因子。在热灭活血清中形成的复合物在引起细胞分泌细胞因子方面与未调理的复合物一样有效。补体调节白细胞分泌细胞因子的能力至少部分与免疫复合物的大小有关，蔗糖密度梯度显示未调理的复合物和溶解的免疫沉淀物大于调理的免疫复合物。相比之下，C4与免疫复合物的液相结合（其不明显改变免疫复合物的大小）显著增加了PBMC的IL-1 β分泌。(C)北京：科学出版社.
We have previously shown that immune complexes isolated from children with juvenile rheumatoid arthritis are heterogeneous in their size, composition, and proinflammatory capacities. The experiments described here mere undertaken to clarify further the roles of size and composition in determining the proinflammatory effects of immune complexes. We incubated peripheral blood mononuclear cells (PBMCs) with different soluble immune complex preparations: opsonized complexes, which were formed in the presence of serum, unopsonized complexes, which were formed in the absence of serum, and immune precipitates solubilized by complement after their formation. ELISA assays showed that immune complexes formed in the presence of complement were less efficient than unopsonized complexes in inducing LL-1 beta and IL-8 secretion from leukocytes. Solubilized immune precipitates showed intermediate capacity to stimulate the release of both cytokines. Complexes formed in heat-inactivated serum were as efficient as unopsonized complexes in eliciting cytokine secretion from the cells. The capacity of complement to regulate cytokine secretion from leukocytes was related, at least in part, to immune complex size, Sucrose density gradients showed unopsonized complexes and solubilized immune precipitates were larger than opsonized immune complexes. In contrast, fluid-phase binding of C4 to immune complexes, which did not appreciably change immune complex size, substantially increased IL-1 beta secretion from PBMC. (C) 1999 Academic Press.