Catabolite control protein A controls hydrogen peroxide production and cell death in Streptococcus sanguinis.
Catabolite control protein A controls hydrogen peroxide production and cell death in Streptococcus sanguinis.
复制标题
分解代谢控制蛋白 A 控制血链球菌中过氧化氢的产生和细胞死亡。
DOI：
10.1128/jb.01131-10
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发表时间：
2011
影响因子：
3.2
通讯作者：
Kreth,Jens
中科院分区：
文献类型：
--
作者：
Zheng,Lanyan;Chen,Zhijun;Itzek,Andreas;Ashby,Michael;Kreth,Jens
血链球菌是一种依赖于丙酮酸氧化酶(Spx)活性而产生过氧化氢(H_2O_2)的共生口腔细菌。除了众所周知的在物种间竞争中的细菌拮抗作用外，过氧化氢还导致约10%的细胞死亡。有血统的人。由于过氧化氢诱导的细胞死亡，大部分完整的染色体DNA被释放到环境中。这种胞外DNA(EDNA)有助于在有氧条件下的自我聚集表型。为了进一步研究spx基因的表达调控，我们评估了分解代谢控制蛋白A(CCPA)对spx基因表达调控的作用。我们在这里报道了CCPA抑制spx的表达。一个同基因的ΔccpA突变体表现出Spx表达上调，Spx丰度增加，过氧化氢产生增加，而野生型在葡萄糖和其他碳水化合物存在下对Spx表达的改变没有反应。由于H_2O_2直接参与EDNA的释放和细菌细胞死亡，目前的数据表明CCPA在这一重要的发育过程中是一个中央控制元件。血统。
Streptococcus sanguinisis a commensal oral bacterium producing hydrogen peroxide (H2O2) that is dependent on pyruvate oxidase (Spx) activity. In addition to its well-known role in bacterial antagonism during interspecies competition, H2O2causes cell death in about 10% of theS. sanguinispopulation. As a consequence of H2O2-induced cell death, largely intact chromosomal DNA is released into the environment. This extracellular DNA (eDNA) contributes to the self-aggregation phenotype under aerobic conditions. To further investigate the regulation ofspxgene expression, we assessed the role of catabolite control protein A (CcpA) inspxexpression control. We report here that CcpA repressesspxexpression. An isogenic ΔccpAmutant showed elevatedspxexpression, increased Spx abundance, and H2O2production, whereas the wild type did not respond with alteredspxexpression in the presence of glucose and other carbohydrates. Since H2O2is directly involved in the release of eDNA and bacterial cell death, the presented data suggest that CcpA is a central control element in this important developmental process inS. sanguinis.