Protein Inactivation by Low-temperature Atmospheric Pressure Plasma in Aqueous Solution
Protein Inactivation by Low-temperature Atmospheric Pressure Plasma in Aqueous Solution
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DOI：
10.1002/ppap.201100063
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发表时间：
2012-01-01
影响因子：
3.5
通讯作者：
Shiraki, Kentaro
中科院分区：
文献类型：
--
作者：
Takai, Eisuke;Kitano, Katsuhisa;Shiraki, Kentaro
等离子体医学是一个很有吸引力的新的研究领域，但与水溶液中的生物大分子的等离子体修饰相关的基本信息仍然是难以捉摸的。如本文所述，我们以溶菌酶为模型研究了低温大气压等离子体对水溶液中蛋白质的化学影响。等离子体处理降低了酶的活性，改变了二级结构，这是由于化学修饰增加了溶菌酶的分子量。这些效应既不是由紫外光也不是由等离子体热引起的，这表明等离子体产生的反应性物质影响溶菌酶。本文提出的信息是阐明生物医学应用中蛋白质等离子体诱导的化学反应的关键的第一步。
Plasma medicine is an attractive new research area, but fundamental information related to plasma modification of biomacromolecules in aqueous solution remains elusive. As described herein, we investigated the chemical effects of low-temperature atmospheric pressure plasma on protein in aqueous solution using lysozyme as a model. Plasma treatment decreased enzymatic activity and changed the secondary structure that results from the increased molecular weight of lysozyme with chemical modification. These effects arise neither from UV light nor from plasma heat, suggesting that the reactive species generated by the plasma affect lysozyme. The information presented in this paper represents a crucial first step for elucidating chemical reactions induced by plasma on proteins for biomedical applications.