Glia-synapse interaction through Ca2+-permeable AMPA receptors in Bergmann glia
Glia-synapse interaction through Ca2+-permeable AMPA receptors in Bergmann glia
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DOI：
10.1126/science.1058827
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发表时间：
2001-05-04
期刊：
影响因子：
56.9
通讯作者：
Ozawa, S
中科院分区：
文献类型：
--
作者：
Lino, M;Goto, K;Ozawa, S
神经胶质细胞表达多种神经递质受体。值得注意的是，小脑中的Bergmann神经胶质细胞具有不含GluR 2亚基的Ca 2+可渗透的α-氨基-3-羟基-5-甲基-4-异恶唑丙酸（AMPA）型谷氨酸受体（AMPAR）。为了阐明这些Ca 2+可渗透的AMPAR的作用，我们通过腺病毒介导的GluR 2基因递送将它们转化为Ca 2+不可渗透的受体。这种转化使蒲肯野细胞树突棘上的神经胶质突起回缩，阻碍突触释放的谷氨酸的清除，并使蒲肯野细胞受到爬行纤维的多重神经支配。因此，神经胶质细胞的钙渗透AMPAR是必不可少的适当的结构和功能之间的关系，伯格曼神经胶质细胞和神经元突触。
Glial cells express a variety of neurotransmitter receptors, Notably, Bergmann glial cells in the cerebellum have Ca2+-permeable alpha -amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA)-type glutamate receptors (AMPARs) assembled without the GluR2 subunit, To elucidate the role of these Ca2+-permeable AMPARs, we converted them into Ca2+-impermeable receptors by adenoviral-mediated delivery of the GluR2 gene. This conversion retracted the glial processes ensheathing synapses on Purkinje cell dendritic spines and retarded the removal of synaptically released glutamate, Furthermore, it caused multiple innervation of Purkinje cells by the climbing fibers. Thus, the glial Ca2+-permeable AMPARs are indispensable for proper structural and functional relations between Bergmann glia and glutamatergic synapses.