In vivo detection of cancer cells with immunoconjugated fluorescent probes by macro zoom microscopy and two-photon microscopy
In vivo detection of cancer cells with immunoconjugated fluorescent probes by macro zoom microscopy and two-photon microscopy
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通过宏观变焦显微镜和双光子显微镜用免疫偶联荧光探针体内检测癌细胞
DOI：
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发表时间：
2015
期刊：
影响因子：
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通讯作者：
Takeshi Imamura
中科院分区：
文献类型：
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作者：
Shigehiro Koga;Y. Oshima;Atsuhiko Hikita;Koichi Sato;Motohira Yoshida;Yuji Yamamoto;T. Iimura;Yuji Watanabe;Takeshi Imamura
我们研制了一种近红外荧光标记的抗癌胚抗原(CEA)抗体，用于CEA表达细胞的荷瘤小鼠，并用宏变焦显微镜进行了体内光学成像。结果，在体内特异性地检测到表达CEA的癌细胞的肿瘤。此外，通过双光子显微镜在体内获得了亚细胞水平的肿瘤特异性荧光图像。在临床前应用中，双光子显微镜也成功地显示了淋巴结微转移。这些结果表明，双光子激发显微镜与免疫标记探针相结合，可广泛应用于临床癌症检测。
We developed a near infrared fluorophore-conjugated anti–Carcinoembryonic antigen (CEA) antibody for mice bearing tumor of CEA expressing cells, and demonstrated in vivo optical imaging by macro zoom microscopy. In the result, tumors of CEA-expressing cancer cells were specifically detected in vivo. Furthermore, cancer-specific fluorescence images were acquired at subcellular level in vivo by two-photon microscopy. In preclinical applications, the lymph node micrometastasis was also successfully visualized by two-photon microscopy. These results suggest that two-photon excitation microscopy in combination with an immunoconjugated probe could be widely adapted to cancer detection in clinical settings.
载体 pEF-BOS、pcDNA1 和 pcDNA3 的修饰提高了便利性和表达能力。
影响因子：
2.7
作者：
Goldman,LA;Cutrone,EC;Kotenko,SV;Krause,CD;Langer,JA
通讯作者：
Langer,JA