Cryoinjury as a myocardial infarction model for the study of cardiac regeneration in the zebrafish
Cryoinjury as a myocardial infarction model for the study of cardiac regeneration in the zebrafish
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DOI：
10.1038/nprot.2012.025
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发表时间：
2012-04-01
期刊：
影响因子：
14.8
通讯作者：
Mercader, Nadia
中科院分区：
文献类型：
--
作者：
Manuel Gonzalez-Rosa, Juan;Mercader, Nadia
斑马鱼的心脏在心室切除后具有再生能力。虽然这种再生模型已被证明有助于阐明某些再生机制，但它是基于去除心脏组织而不是组织损伤。我们最近的特点是细胞反应和再生能力的斑马鱼心脏冷冻损伤（CI）后，一种替代的程序，更密切地模拟了人类心脏心肌梗死（MI）后所经历的病理生理过程。麻醉后，用液氮冷却的铜探针进行局部CI，导致25%的心室损伤，
The zebrafish heart has the capacity to regenerate after ventricular resection. Although this regeneration model has proved useful for the elucidation of certain regeneration mechanisms, it is based on the removal of heart tissue rather than on tissue damage. We recently characterized the cellular response and regenerative capacity of the zebrafish heart after cryoinjury (CI), an alternative procedure that more closely models the pathophysiological process undergone by the human heart after myocardial infarction (MI). After anesthesia, localized CI with a liquid nitrogen-cooled copper probe induced damage in 25% of the ventricle, in a procedure requiring