Monitoring HSV-TK/ganciclovir cancer suicide gene therapy using CdTe/CdS core/shell quantum dots
Monitoring HSV-TK/ganciclovir cancer suicide gene therapy using CdTe/CdS core/shell quantum dots
复制标题
使用 CdTe/CdS 核/壳量子点监测 HSV-TK/更昔洛韦癌症自杀基因治疗
DOI：
10.1016/j.biomaterials.2012.02.058
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发表时间：
2012-06-01
期刊：
影响因子：
14
通讯作者：
Zhang, Hong
中科院分区：
文献类型：
--
作者：
Shao, Dan;Zeng, Qinghui;Zhang, Hong
能够标记基因并监测其迁移是肿瘤自杀基因治疗临床应用的关键。本文介绍了光子纳米材料。利用EDC/NHS偶联方法成功地将单纯疱疹病毒胸苷激酶（HSV-TK）基因与CdTe/CdS核壳量子点（QDs）连接在一起。从共聚焦显微镜，它被证明，质粒TK细胞内的运输可以有效地和明确地跟踪通过监测发光的量子点高达96小时后转染到Hela细胞的量子点-TK缀合物。MTT结果表明，GCV对QDs-TK偶联物具有很强的细胞毒作用，而QDs对Hela细胞的生长过程无明显影响。量子点-TK偶联物诱导的细胞凋亡被清晰地追踪，部分原因是量子点的强发光。我们的研究结果表明，光子纳米材料，如量子点，提供了一个工具，用于监测TK基因的传递和抗癌活性。(C)2012爱思唯尔有限公司保留所有权利。
To be able to label a gene and monitor its migration are key important approaches for the clinical application of cancer suicide gene therapy. Photonic nanomaterials are introduced in this work. One of the most promised suicide genes - herpes simplex virus thymidine kinase (HSV-TK) gene - is successfully linked with CdTe/CdS core/shell quantum dots (QDs) via EDC/NHS coupling method. From confocal microscopy it was demonstrated that plasmid TK intracellular trafficking can be effectively and distinctly traced via monitoring the luminescence of the QDs up to 96 h after transfection of QDs-TK conjugates into Hela cells. MTT results show that the QDs-TK conjugates have a high efficient cytotoxicity after adding GCV into Hela cells, whereas the QDs exert no detectable deleterious effects on the cellular processes. The apoptosis induced by QDs-TK conjugates with GCV is distinctly traced partly due to the strong luminescence of the QDs. Our results indicate that photonic nanomaterials, e.g. QDs, provide a tool for monitoring TK gene delivery and anti-cancer activity. (C) 2012 Elsevier Ltd. All rights reserved.