Neurite outgrowth in molluscan organ and cell cultures: the role of conditioning factor(s)
Neurite outgrowth in molluscan organ and cell cultures: the role of conditioning factor(s)
复制标题
软体动物器官和细胞培养物中的神经突生长：调节因子的作用
作者：
R. G. Wong;R. Hadley;S. Kater;G. Hauser
用改良的Liebowitz L-15培养基体外培养螺体中央神经节神经元。这些神经元显示出与急性解剖神经节相当的电兴奋性。分离的神经元在整个培养持续时间长达2周的确定培养基中保持球形。这种静态的形态形成对比的显着神经炎的生长发生在培养基中与共培养的完整的Helisoma脑或脑条件培养基中的神经元。生长锥形成和神经突延伸的形态序列仅在调节因子存在下发生，其作用模式包括调节因子与基质的紧密结合。在这些条件下，两个主要的神经元表型，电兴奋性和复杂的神经元结构，可以在体外培养的成年软体动物神经元独立的影响。
Isolated neurons from adult central ganglia of the snail Helisoma were cultured in vitro in modified Liebowitz L-15 medium. Such neurons displayed electrical excitability comparable to that in acutely dissected ganglia. Isolated neurons remained spherical in defined medium throughout culture durations up to 2 weeks. This static morphology was contrasted by the significant neuritic outgrowth which occurred from neurons maintained in medium with co-cultured intact Helisoma brains or in brain conditioned medium. A morphological sequence of growth cone formation and neurite extension occurred only in the presence of a conditioning factor(s) with a mode of action which included tight binding of the conditioning factor to the substratum. Under these conditions, the two primary neuronal phenotypes, electrical excitability and complex neuronal architecture, could be affected independently in adult molluscan neurons cultured in vitro.