Characteristics of GABAergic neurons in the superficial superior colliculus in mice
Characteristics of GABAergic neurons in the superficial superior colliculus in mice
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DOI：
10.1016/s0304-3940(03)00570-6
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发表时间：
2003-07-31
影响因子：
2.5
通讯作者：
Isa, T
中科院分区：
文献类型：
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作者：
Endo, T;Yanagawa, Y;Isa, T
我们利用绿色荧光蛋白(GFP)特异性表达的转基因小鼠，研究了浅上丘(SSC)切片中已鉴定的GABA能神经元的特征。全细胞膜片钳记录和细胞内生物细胞素染色显示GFP阳性神经元的电生理和形态特征。GFP阳性神经元的树突野呈切线状延长。对去极化电流脉冲的反应，大多数GFP阳性神经元表现出两种放电特性中的一种：55%(n=36/65)以恒定的放电频率产生连续的棘波，22%(n=14/65)的神经元以产生棘波的阈值水平进行瞬时爆发放电。这些结果阐明了GABA能神经元的功能异质性。为了解GABA能系统在SSC中的功能提供了基础信息。(C)2003爱思唯尔爱尔兰科学有限公司。保留所有权利。
We investigated characteristics of identified GABAergic neurons in slices of the superficial superior colliculus (sSC) using transgenic mice in which green fluorescent protein (GFP) is specifically expressed in GABAergic neurons. Whole-cell patch clamp recordings and intracellular staining with biocytin revealed electrophysiological and morphological properties of GFP-positive neurons. GFP-positive neurons had a tangentially elongated wide dendritic field. In response to depolarizing current pulses, most of the GFP-positive neurons showed either of two firing properties; 55% (n = 36/65) generated successive spikes with a constant firing frequency and 22% (n = 14/65) showed transient burst firing at a threshold level for spike generation. These results elucidate a functional heterogeneity of GABAergic neurons. and provide fundamental information to understand the function of the GABAergic system in the sSC. (C) 2003 Elsevier Science Ireland Ltd. All rights reserved.