Chicken bone marrow-derived dendritic cells maturation in response to infectious bursal disease virus
Chicken bone marrow-derived dendritic cells maturation in response to infectious bursal disease virus
复制标题
鸡骨髓来源的树突状细胞成熟以应对传染性法氏囊病病毒。
DOI：
10.1016/j.vetimm.2014.12.012
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发表时间：
2015-03-15
影响因子：
1.8
通讯作者：
Yang, Qian
中科院分区：
文献类型：
--
作者：
Liang, Jinfeng;Yin, Yinyan;Yang, Qian
传染性法氏囊病病毒（IBDV）是一种高度传染性疾病，易导致雏鸡免疫抑制和免疫应答下降。由于树突状细胞（DC）是诱导免疫的关键细胞，其成熟和功能受微生物和环境刺激的影响，我们研究了灭活的IBDV和IBDV对鸡DC激活和成熟的影响。在完全培养基中培养的鸡骨髓源性树突状细胞（chBM-DCs）（包括重组鸡：粒细胞-巨噬细胞集落刺激因子和白细胞介素4）表达高水平的MHC-II和CD11c。经LPS或病毒刺激后，chbm - dc表现出典型的dc形态。此外，与未受刺激的chBM-DCs相比，LPS或病毒刺激显著提高了chBM-DCs表面CD40和CD86分子的表达水平，以及诱导t细胞增殖反应的能力。有趣的是，与活的IBDV相比，失活的IBDV表现出更强的上调CD40和CD86分子表达水平和刺激幼稚T细胞增殖的能力。这些结果表明，活病毒感染会损害DC的成熟和功能，这可能解释了为什么感染IBDV的鸡不能触发有效的特异性免疫反应或发展免疫记忆。(C) 2015 Elsevier B.V.版权所有
Infectious bursal disease virus (IBDV) is highly contagious disease which easily lead to immunosuppression and a decreased response to vaccinations in young chicken. Since dendritic cells (DCs) are crucial to induce immunity and their maturation and functions are influenced by microbial and environmental stimuli, we investigated the effects of inactivated IBDV and IBDV on chicken DC activation and maturation. Chicken bone marrow-derived dendritic cells (chBM-DCs) cultured in complete medium (including recombinant chicken: granulocyte-macrophage colony-stimulating factor and interleukin 4) expressed high levels of MHC-II and the putative CD11c. After LPS or virus stimulation, chBM-DCs displayed the typical morphology of DCs. In addition, stimulation by LPS or viruses significantly elevated chBM-DCs surface expression levels of CD40 and CD86 molecules, as well as the ability to induce T-cell proliferative response, compared to the non-stimulated chBM-DCs. Interestingly, inactive IBDV showed stronger ability to up-regulate expression levels of CD40 and CD86 molecules and stimulate naive T cells proliferation than live IBDV. These results revealed that live viruses infection impaired DC maturation and functions, probably explaining why chickens infected with IBDV fails to trigger an effective specific immune response or develop immune memory. (C) 2015 Elsevier B.V. All rights reserved.