A chromogenic substrate culture plate for early identification of Vibrio vulnificus and isolation of other marine Vibrios.
A chromogenic substrate culture plate for early identification of Vibrio vulnificus and isolation of other marine Vibrios.
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发表时间：
2007-09
影响因子：
0.8
通讯作者：
Yukari Nakashima;Megumi Oho;K. Kusaba;Z. Nagasawa;O. Komatsu;Isao Manome;Kazukuni Araki;Hirotaka Oishi;Mikio Nakashima
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作者：
Yukari Nakashima;Megumi Oho;K. Kusaba;Z. Nagasawa;O. Komatsu;Isao Manome;Kazukuni Araki;Hirotaka Oishi;Mikio Nakashima
创伤弧菌感染可导致坏死性筋膜炎和败血症，潜伏期短，死亡率高。因此，需要一种简单快速的检测方法来改善结果。为了将创伤弧菌与引起坏死性筋膜炎的其他病原体区分开来，我们开发了一种选择性分离培养琼脂平板（Chromochecker Vibrio Agar-1；CVA-1），用于环境监测和临床环境。已通过生化鉴定的 104 株创伤弧菌菌株在 CVA-1 上生长时显示出典型的菌落形态和颜色。 CVA-1上疑似创伤弧菌的51份海洋细菌样本中的36份随后通过生化鉴定系统鉴定为创伤弧菌。在已知引起坏死性筋膜炎的 8 种细菌中，只有创伤弧菌在 CVA-1 上生长。此外，CVA-1 上的生长使弧菌物种易于分化。 CVA-1可根据菌落形态和染色差异来区分致病性弧菌。
Vibrio vulnificus infection can result in necrotizing fasciitis and sepsis, which have short latentcy periods and high mortality rates. Thus, an easy and quick detection method is needed to improve the outcome. To distinguish V. vulnificus from other pathogens that cause necrotizing fasciitis, we developed a selective isolation culture agar plate (Chromochecker Vibrio Agar-1; CVA-1) for use in environmental monitoring and in the clinical setting. One hundred four strains of V. vulnificus, already identified biochemically, showed typical colony form and color when grown on CVA-1. Thirty-six of 51 marine bacteria samples suspected to be V. vulnificus on CVA-1 were subsequently identified as V. vulnificus by a biochemical identification system. Of 8 bacteria known to cause necrotizing fasciitis, only V. vulnificus grew on CVA-1. In addition, growth on CVA-1 allowed ready differentiation of Vibrio species. CVA-1 can be used to distinguish pathogenic Vibrios according to colony form and chromatic differences.