Antibacterial activity and cytotoxicity of two novel cross-linking antibacterial monomers on oral pathogens
Antibacterial activity and cytotoxicity of two novel cross-linking antibacterial monomers on oral pathogens
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两种新型交联抗菌单体对口腔病原体的抗菌活性和细胞毒性
DOI：
10.1016/j.archoralbio.2010.10.011
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发表时间：
2011-04-01
影响因子：
3
通讯作者：
Chen, Ji-hua
中科院分区：
文献类型：
--
作者：
Huang, Li;Xiao, Yu-hong;Chen, Ji-hua
目的：本研究测试了两种新型交联抗菌单体2-甲基丙烯酰氧基乙基十二烷基甲基溴化铵（MAE-DB）和2-甲基丙烯酰氧基乙基十六烷基甲基溴化铵（MAE-HB）的抗菌活性和细胞毒性。使用肉汤稀释试验测试了 MAE-HB 对八种口腔细菌菌株的抵抗力。进行时间杀灭测定以检查未聚合的 MAE-DB 和 MAE-HB 对抗变形链球菌 UA159 和血链球菌 ATCC6715 的动力学。使用场发射扫描电子显微镜（Fe-SEM）观察细菌形态。使用甲基噻唑基四唑试验评估未聚合的两种新单体和 Bis-GMA 对人牙龈成纤维细胞系 H2620 的细胞毒性。结果：未聚合的 MAE-DB 和 MAE-HB 对口腔细菌表现出很强的杀菌活性。 MAE-DB的MBC值范围为12.2至24.4μg/ml，MAE-HB的MBC值范围为6.2至48.8μg/ml。时间杀灭测定表明，未聚合的MAE-DB和MAE-HB在4x MBC浓度下对变异链球菌UA159和血链球菌ATCC6715具有快速杀灭作用。 Fe-SEM观察表明MAE-DB和MAE-HB会扰乱细菌的完整性并导致细菌细胞裂解。两种单体对人牙龈成纤维细胞的中位致死浓度值均在10～20μg/ml之间，均高于Bis-GMA。结论：未聚合的MAE-DB和MAE-HB单体对8种口腔细菌均具有较强的杀菌活性。它们的细胞毒性低于 Bis-GMA。 (C) 2010 Elsevier Ltd. 保留所有权利。
Objectives: The antibacterial activity and cytotoxicity of two novel cross-linking antibacterial monomers, 2-methacryloxylethyl dodecyl methyl ammonium bromide (MAE-DB) and 2-methacryloxylethyl hexadecyl methyl ammonium bromide (MAE-HB) were tested in this study.Design: The minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) of unpolymerized MAE-DB and MAE-HB against eight strains of oral bacteria were tested using a broth dilution test. Time-kill determinations were performed to examine the kinetics of unpolymerized MAE-DB and MAE-HB against Streptococcus mutans UA159 and Streptococcus sanguinis ATCC6715. Bacterial morphology was observed using a field emission scanning electron microscope (Fe-SEM). The cytotoxicity of unpolymerized two new monomers and Bis-GMA on the human gingival fibroblast cell line H2620 was assessed using a methyl thiazolyl tetrazolium assay.Results: Unpolymerized MAE-DB and MAE-HB showed strong bactericidal activity against oral bacteria. The MBC value of MAE-DB ranged from 12.2 to 24.4 mu g/ml and the MBC value of MAE-HB ranged from 6.2 to 48.8 mu g/ml. Time-kill determinations indicated that unpolymerized MAE-DB and MAE-HB had rapid killing effects against S. mutans UA159 and S. sanguinis ATCC6715 at the concentration of 4x MBC. The Fe-SEM observation showed that MAE-DB and MAE-HB could disturb the integrity of bacteria and cause lysis of bacterial cells. The median lethal concentration values on human gingival fibroblast for both monomers were between 10 and 20 mu g/ml, and greater than that of Bis-GMA.Conclusions: Unpolymerized MAE-DB and MAE-HB monomers had strong bactericidal activity against eight strains of oral bacteria. Their cytotoxicities were less than that of Bis-GMA. (C) 2010 Elsevier Ltd. All rights reserved.