Individual mice can be distinguished by the period of their islet calcium oscillations - Is there an intrinsic islet period that is imprinted in vivo?
Individual mice can be distinguished by the period of their islet calcium oscillations - Is there an intrinsic islet period that is imprinted in vivo?
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DOI：
10.2337/diabetes.54.12.3517
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发表时间：
2005-12-01
期刊：
影响因子：
7.7
通讯作者：
Satin, LS
中科院分区：
文献类型：
--
作者：
Nunemaker, CS;Zhang, M;Satin, LS
据信，体内脉冲式胰岛素分泌部分源自个体胰岛的固有葡萄糖依赖性细胞内钙浓度（[Ca2 +]（i））脉动性。在孤立的情况下，胰岛显示快速、缓慢或快速和缓慢[Ca2 +]（i）振荡的混合。我们发现，胰岛[Ca2 +]（i）振荡的周期对每只小鼠都是独特的，来自单个小鼠的胰岛表现出彼此相似的节律。基于它们的节律周期，将小鼠大致分类为快（0.65 +/-0.1分钟; n = 6只小鼠）或慢（4.7 +/-0.2分钟; n = 15只小鼠）。为了确保这种现象不是胰岛间交流的假象，我们证实了分离培养的胰岛（周期：2.9 +/-0.1分钟）与来自同一只小鼠的一起培养的胰岛（3.1 +/-0.1分钟，P> 0.52，n = 5只小鼠）没有统计学差异。我们还比较了在体内测量的脉动胰岛素模式与在体外测量的6只小鼠的胰岛[Ca 2 +]（i）模式。具有较快胰岛素脉冲周期的小鼠对应于较快的胰岛[Ca2 +]（i）模式，而较慢的胰岛素模式对应于较慢的[Ca2 +]（i）模式，这表明每只小鼠的胰岛素节律在一定程度上由其体外胰岛保留。我们建议，个别小鼠具有特征振荡[Ca2 +]（i）模式，这是通过一个未知的机制在体内印迹。
Pulsatile insulin secretion in vivo is believed to be derived, in part, from the intrinsic glucose-dependent intracellular calcium concentration ([Ca2+](i)) pulsatility of individual islets. In isolation, islets display fast, slow, or mixtures of fast and slow [Ca2+](i) oscillations. We show that the period of islet [Ca2+](i) oscillations is unique to each mouse, with the islets from an individual mouse demonstrating similar rhythms to one another. Based on their rhythmic period, mice were broadly classified as being either fast (0.65 +/- 0.1 min; n = 6 mice) or slow (4.7 +/- 0.2 min; n = 15 mice). To ensure this phenomenon was not an artifact of islet-to-islet communication, we confirmed that islets cultured in isolation (period: 2.9 +/- 0.1 min) were not statistically different from islets cultured together from the same mouse (3.1 +/- 0.1 min, P > 0.52, n = 5 mice). We also compared pulsatile insulin patterns measured in vivo with islet [Ca2+](i) patterns measured in vitro from six mice. Mice with faster insulin pulse periods corresponded to faster islet [Ca2+](i) patterns, whereas slower insulin patterns corresponded to slower [Ca2+](i) patterns, suggesting that the insulin rhythm of each mouse is preserved to some degree by its islets in vitro. We propose that individual mice have characteristic oscillatory [Ca2+](i) patterns, which are imprinted in vivo through an unknown mechanism.