Mesoporous silica nanoparticles incorporated hybrid monolithic stationary phase immobilized with pepsin for enantioseparation by capillary electrochromatography
Mesoporous silica nanoparticles incorporated hybrid monolithic stationary phase immobilized with pepsin for enantioseparation by capillary electrochromatography
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DOI：
10.1016/j.jpba.2017.03.013
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发表时间：
2017-06
影响因子：
3.4
通讯作者：
Shujuan Xu;Rongzhen Mo;can Jin;Xiaoqin Cui;Ruihan Bai;Yibing Ji
中科院分区：
文献类型：
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作者：
Shujuan Xu;Rongzhen Mo;can Jin;Xiaoqin Cui;Ruihan Bai;Yibing Ji
本研究开发了一种新型的手性杂化单片固定相介孔二氧化硅纳米颗粒。首先用氨基修饰的介孔二氧化硅纳米颗粒（NH2-MSN）、甲基丙烯酸缩水甘油酯（GMA）和二甲基丙烯酸乙烯（EDMA）原位共聚制备了固定相，然后用胃蛋白酶作为手性选择剂进行了功能化。掺入的介孔二氧化硅纳米颗粒提供了额外的相互作用位点，从而产生了不同的对映体选择性，从而增强了整体分离。该柱成功地用于毛细管电色谱法对15种碱性手性药物的对映体分离。考察了纳米颗粒含量、胃蛋白酶浓度、缓冲液pH、外加电压等因素的影响。所有分析物均可在10分钟内洗脱，其中9个可达到基线分离。通过日内、日间、柱对柱和批对批调查获得了令人满意的重复性，相对标准偏差小于4.2%。这些结果表明，同时利用介孔二氧化硅纳米颗粒的独特性质和整体材料的通用性可能是一种有前途的对映体分离策略。
In this study, a novel mesoporous silica nanoparticles incorporated chiral hybrid monolithic stationary phase was developed. The stationary phase was firstly prepared by an in situ copolymerization of amino-modified mesoporous silica nanoparticles (NH2-MSN), glycidyl methacrylate (GMA), and ethylene dimethacrylate (EDMA) and then functionalized with pepsin as chiral selector. The incorporated mesoporous silica nanoparticles provided additional interactions sites, and in turn yielded different enantioselectivity thus enhancing the overall separation. The column was successfully employed for enantioseparation of fifteen basic chiral drugs in capillary electrochromatography. Effects of nanoparticles percentage, pepsin concentration, the pH of running buffer and the applied voltage were investigated. All the analytes could be eluted in less than ten minutes and nine of them could achieve baseline separation. Satisfactory repeatabilities with relative standard deviations less than 4.2% were achieved through intraday, interday, column-to-column and batch-to-batch investigations. These results indicated that the simultaneous utilization of the unique properties of mesoporous silica nanoparticles and versatile features of monoliths could be a promising strategy for enantioseparation.