Antibody-dependent lymphocyte cytotoxicity in the murine sarcoma virus system: activity of IgM and IgG with specificity for MLV determined antigen(s).
Antibody-dependent lymphocyte cytotoxicity in the murine sarcoma virus system: activity of IgM and IgG with specificity for MLV determined antigen(s).
复制标题
鼠肉瘤病毒系统中抗体依赖性淋巴细胞的细胞毒性：IgM 和 IgG 的活性，对 MLV 确定的抗原具有特异性。
作者：
E. Lamon;H. Skurzak;B. Andersson;H. Whitten;E. Klein
在微细胞毒性试验中，研究了具有Moloney白血病病毒（MLV）特异性抗原的抗血清诱导携带MLV抗原的淋巴细胞减少目标细胞的能力。研究发现，退行性莫洛尼肉瘤病毒（MSV）肿瘤动物的血清和进行性生长的MSV肿瘤动物的血清可诱导正常淋巴细胞对靶标产生活性。回归因子血清在感染MSV后15天和回归因子血清在感染MSV后50天对荷瘤动物的免疫淋巴细胞产生细胞毒性。抗血清的19S和7S Sephadex G-200组分均能诱导正常淋巴细胞的细胞毒性，并增强对MSV免疫淋巴细胞的细胞毒性。通过sepharose偶联抗小鼠IgM和抗小鼠IgG柱，分别证明了这些活性是IgM和IgG。通过在两列上传递血清来去除所有活性。
Antisera with specificity for Moloney leukemia virus-(MLV) determined antigen(s) were studied for their ability to induce MLV antigen bearing target cell reduction by lymphocytes in microcytotoxicity assays. Sera from animals which had regressed Moloney sarcoma virus (MSV) tumors as well as sera from animals with progressively growing MSV tumors were found to induce normal lymphocytes to be active against the targets. Regressor serum was found also to induce cytotoxicity by immune lymphocytes from a tumor-bearing animal 15 days after MSV and from a regressor 50 days after MSV infection. Both the 19S and 7S Sephadex G-200 fractions of the antisera were found to induce cytotoxicity by normal lymphocytes and to potentiate the cytotoxicity of MSV immune lymphocytes. These activities were shown to be IgM and IgG, respectively, by the use of Sepharose-coupled anti-mouse IgM and anti-mouse IgG columns. All activity was removed by passing sera over both columns.