Breeding for highly fertile isolates of Nectria haematococca MPVI that are highly virulent on pea and in planta selection for virulent recombinants
Breeding for highly fertile isolates of Nectria haematococca MPVI that are highly virulent on pea and in planta selection for virulent recombinants
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DOI：
10.1094/phyto.2001.91.1.92
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发表时间：
2001-01-01
期刊：
影响因子：
3.2
通讯作者：
VanEtten, HD
中科院分区：
文献类型：
--
作者：
Funnell, DL;Matthews, PS;VanEtten, HD
异宗配合子囊菌红球藻交配群体VI（无性型茄病镰刀菌）是一种寄主范围广的病原体。该真菌的田间分离株对豌豆是致病的，往往是雌性不育的，低生育力，和相同的交配型（MAT-I），而雌性可育分离株的交配型是高生育力的，往往是非致病的这种植物。为了促进可能是重要的能力，N，红球藻寄生豌豆的性状的遗传分析，进行了育种项目，以产生两性分离的每一个交配类型，是高度肥沃和高度致命的豌豆。虽然关联的高毒力豌豆与女性不育和MAT-I交配型没有完全打破，具有高生育力和高毒力豌豆菌株在两代内繁殖。还通过另一种方法分离高毒性后代，其中用来自两个中等毒性亲本之间的杂交的子囊孢子的混合物接种豌豆植物。而所有的子囊孢子分离没有选择在植物比父母的毒性较低，从患病组织中回收的许多菌株比父母的菌株更具毒性。限制性片段长度多态性分析表明，一些回收的分离物是亲本的遗传重组体，表明豌豆组织选择毒性重组体。所有测试的高毒性分离物都具有使豌豆植物抗毒素pisatin解毒的能力，再次显示了该性状与豌豆上的致病性之间的联系。
The heterothallic ascomycete Nectria haematococca mating population VI (anamorph Fusarium solani) is a broad host range pathogen. Field isolates of this fungus that are pathogenic on pea tend to be female sterile, of low fertility, and the same mating type (MAT-I), whereas female fertile isolates of either mating type that are highly fertile tend to be nonpathogenic on this plant. To facilitate genetic analysis of traits that may be important in the ability of N, haematococca to parasitize peas, a breeding project was undertaken to produce hermaphroditic isolates of each mating type that are highly fertile and highly virulent on peas. Although the association of high virulence on peas with female sterility and the MAT-I mating type was not completely broken, isolates with high fertility and high virulence on peas were bred within two generations. Highly virulent progeny were also isolated by an alternative method in which pea plants were inoculated with a mixture of ascospores from a cross between two moderately virulent parents. Whereas all ascospores isolated without selection in planta had lower virulence than the parents, many isolates recovered from diseased tissue were more virulent than the parental isolates. Some of the recovered isolates were shown by restriction fragment length polymorphism analysis to be genetic recombinants of the parents, demonstrating that the pea tissue selected virulent recombinants. All highly virulent isolates tested had the ability to detoxify the pea phytoalexin pisatin, again showing a link between this trait and pathogenicity on the pea.