Calcium spiking activity and baseline calcium levels in ROS 17/2.8 cells exposed to extremely low frequency electromagnetic fields (ELF EMF).
Calcium spiking activity and baseline calcium levels in ROS 17/2.8 cells exposed to extremely low frequency electromagnetic fields (ELF EMF).
复制标题
暴露于极低频电磁场 (ELF EMF) 的 ROS 17/2.8 细胞中的钙峰值活性和基线钙水平。
DOI：
10.1080/09553000010013779
复制
发表时间：
2001
影响因子：
2.6
通讯作者：
Sisken,JE
中科院分区：
文献类型：
--
作者：
Shahidain,R;Mullins,RD;Sisken,JE
目的确定极低频电磁场是否可以改变平均游离胞质钙离子浓度 [Ca 2+ ] i 以及 ROS 17/2.8 细胞群中 [Ca 2+ ] i 的瞬时增加。 材料和方法将负载有钙选择性发光蛋白、水母发光蛋白的细胞放置在样品室的底部，将样品室插入先前描述的气隙反应器系统的间隙中，在该系统中将细胞暴露于各种频率和通量密度或假条件。获得了由于水母发光蛋白发光而产生的光子计数的实时记录，并使用概率图对数据进行了分析。结果从暴露于各种磁场的细胞获得的数据的概率图实际上与暴露前和暴露后假或无场期间相同培养物获得的数据重叠。结论这些实验没有提供 ELF EMF 对平均 [Ca 2+ ] i 或平均 [Ca 2+ ] i 或[Ca 2+ ] i 短暂增加。
PurposeTo determine whether extremely low frequency electromagnetic fields can alter average free cytosolic calcium ion concentrations [Ca 2+ ] i and transient increases in [Ca 2+ ] i in populations of ROS 17/2.8 cells.Materials and methodsCells loaded with the calcium-selective luminescent photoprotein, aequorin, were placed in the bottom of a sample chamber, which was inserted into the gap of a previously described air gap reactor system where they were exposed either to sinusoidal magnetic fields at a variety of frequencies and flux densities or to sham conditions. Real-time recordings of photon counts due to aequorin luminescence were obtained and data were analysed with the use of probit plots.ResultsProbit plots of data obtained from cells exposed to the various magnetic fields were virtually superimposable over the data obtained for the same cultures during pre- and post-exposure sham or no-field periods.ConclusionThese experiments provided no evidence for any effects of ELF EMF, either positive or negative, on either average [Ca 2+ ] i or on transient increases in [Ca 2+ ] i.