Downregulation of long non-coding RNAs JPX and XIST is associated with the prognosis of hepatocellular carcinoma
Downregulation of long non-coding RNAs JPX and XIST is associated with the prognosis of hepatocellular carcinoma
复制标题
DOI：
10.1016/j.clinre.2016.09.002
复制
发表时间：
2017-03-01
影响因子：
2.7
通讯作者：
Yuan, Yufeng
中科院分区：
文献类型：
--
作者：
Ma, Weijie;Wang, Haitao;Yuan, Yufeng
背景：长链非编码RNA XIST及其激活子JPX在肝细胞癌（HCC）中的表达谱和生物学相关性尚不清楚。我们测量了JPX和XIST在HCC中的表达水平，评估了它们在HCC进展中的临床意义，并验证了它们作为HCC诊断的生物标志物的潜力。方法：采用定量逆转录- pcr （qRT-PCR）技术检测68例肝癌组织及癌旁正常组织中JPX和XIST的表达；分析其与病理特征和总生存率的关系。采用qRT-PCR检测了42例HCC患者和68例健康对照者的血浆JPX/XIST水平，以确定其作为生物标志物的潜力。结果：JPX和XIST水平在HCC中显著降低，并与组织学分级和肿瘤淋巴结转移分期相关（P < 0.05）。JPX和XIST的低表达导致HCC的总生存率明显降低。多因素Cox回归分析显示，JPX/XIST表达水平是影响HCC总生存率的独立预后因素。此外，患者血浆中JPX水平低于对照组；JPX在受试者工作特征曲线下的面积为0.814，JPX与AFP联合使用对HCC患者和对照组的鉴别能力增强（AUC 0.905，特异性72.2%，敏感性97.1%）。结论：JPX和XIST的下调可能是HCC预后不良的新的生物标志物。(C) 2016 Elsevier Masson SAS。版权所有。
Background: The expression profiles and biological relevance of long non-coding RNA XIST and its activator JPX in hepatocellular carcinoma (HCC) are not well elucidated. We measured JPX and XIST expression levels in HCC, evaluated their clinical significance in HCC progression, and verified their potential as biomarkers for diagnosing HCC.Methods: JPX and XIST expression in 68 HCC tissues and adjacent normal tissues were evaluated by quantitative reverse transcription-PCR (qRT-PCR); their association with pathologic features and overall survival was analyzed. Plasma JPX/XIST levels in 42 patients with HCC and 68 healthy controls were measured by qRT-PCR to determine their potential as biomarkers.Results: JPX and XIST levels were significantly decreased in HCC and associated with histological grade and tumor-node-metastasis stage (P < 0.05). Low JPX and XIST expression resulted in significantly poor overall survival of HCC. Multivariate Cox regression analysis demonstrated that JPX/XIST expression levels were independent prognostic factors for HCC overall survival rates. Moreover, plasma JPX levels in patients were lower than that in controls; JPX yielded an area under the receiver operating characteristic curve of 0.814 and the combination of JPX and AFP possessed a promoted ability for discrimination between HCC patients and controls (AUC 0.905, 72.2% specificity, 97.1% sensitivity).Conclusions: Downregulated JPX and XIST may serve as novel biomarkers of poor prognosis in HCC. (C) 2016 Elsevier Masson SAS. All rights reserved.