Spontaneous lipid peroxidation and production of hydrogen peroxide and superoxide in human spermatozoa. Superoxide dismutase as major enzyme protectant against oxygen toxicity.
Spontaneous lipid peroxidation and production of hydrogen peroxide and superoxide in human spermatozoa. Superoxide dismutase as major enzyme protectant against oxygen toxicity.
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DOI：
10.1002/j.1939-4640.1987.tb00973.x
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发表时间：
1987-09
影响因子：
--
通讯作者：
J. Alvarez;J. Touchstone;L. Blasco;B. Storey
中科院分区：
文献类型：
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作者：
J. Alvarez;J. Touchstone;L. Blasco;B. Storey
洗涤后的人类精子中的自发脂质过氧化是通过 32°C 的有氧培养诱导的，并通过丙二醛的产生进行测量；同时测定孵育期间运动性的损失。完全丧失活力时的丙二醛产量（定义为脂过氧化致死终点（LLE））为0.10 +/- 0.03 nmol/10(8) 个细胞（平均值+/- SD，n = 40），并且与完全丧失活力的时间无关。人类精子产生 H2O2 和 O2-。需氧孵化期间。用 KCN 抑制这些细胞中的超氧化物歧化酶表明所有 H2O2 的产生都是由于歧化酶的作用。单个人类精子样本的超氧化物歧化酶活性在 1 至 10 U/10(8) 细胞之间变化，来自单个捐献者的样本之间的差异几乎与不同捐献者之间的差异一样大。完成动力丧失的时间 (tL) 在样品之间显示出 1 至 10 小时的相同变化。对于给定的精子样品，自发脂质过氧化速率（以 LLE/tL 计算）和有氧孵育前测定的同一样品的超氧化物歧化酶活性具有良好的线性相关性（r = 0.97）。谷胱甘肽还原酶、谷胱甘肽过氧化物酶和谷胱甘肽被发现存在于人类精子中，但样本之间的差异很小。这些结果表明超氧化物歧化酶在保护人类精子免受脂质过氧化作用中发挥着主要作用。此外，新鲜精子样本的超氧化物歧化酶活性似乎可以很好地预测该特定样本的寿命（直至完全丧失活力），因此可能在精液分析中有用。
Spontaneous lipid peroxidation in washed human spermatozoa was induced by aerobic incubation at 32 C and measured by malonaldehyde production; loss of motility during the incubation was determined simultaneously. Malonaldehyde production at the point of complete loss of motility, defined as the lipoperoxidative lethal endpoint (LLE), was 0.10 +/- 0.03 nmol/10(8) cells (mean +/- SD, n = 40), and was independent of the time to complete loss of motility. Human spermatozoa produced both H2O2 and O2-. during aerobic incubation. Inhibition of superoxide dismutase in these cells with KCN showed that all the H2O2 production is due to action of the dismutase. The superoxide dismutase activity of individual human sperm samples varied between 1 and 10 U/10(8) cells, variations between samples from a single donor being nearly as great as those between different donors. The time to complete motility loss (tL) showed equal variation of 1 to 10 hours among samples. The rate of spontaneous lipid peroxidation, calculated as LLE/tL, for a given sperm sample and the superoxide dismutase activity of the same sample, determined prior to aerobic incubation, gave a good linear correlation (r = 0.97). Glutathione reductase, glutathione peroxidase, and glutathione were found to be present in human spermatozoa, but showed little variation among samples. These results suggest that superoxide dismutase plays the major role in protecting human spermatozoa against lipid peroxidation. In addition, the superoxide dismutase activity of a fresh sperm sample appears to be a good predictor of the lifetime (up to the complete loss of motility) of that particular sample, and so may prove useful in semen analysis.