An Inducible System for Rapid Degradation of Specific Cellular Proteins Using Proteasome Adaptors.
An Inducible System for Rapid Degradation of Specific Cellular Proteins Using Proteasome Adaptors.
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DOI：
10.1371/journal.pone.0152679
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Matouschek A
中科院分区：
文献类型：
--
作者：
Wilmington SR;Matouschek A
研究蛋白质功能的一种常见方法是从细胞中去除感兴趣的蛋白质并观察其反应。传统的方法涉及干扰基因表达，但这些技术只对新合成的蛋白质有效，而不影响以前存在的和稳定的蛋白质。在这里，我们介绍了一种技术，通过将特定蛋白质以泛素不依赖的方式运送到蛋白酶体进行降解，从而在哺乳动物细胞中诱导特定蛋白质的快速降解。我们介绍了该系统在人类培养细胞中的两种实现方式，这两种方式可以单独用于控制蛋白质浓度。我们的研究提供了一个简单、健壮和灵活的技术平台来操纵细胞内蛋白质水平。
A common way to study protein function is to deplete the protein of interest from cells and observe the response. Traditional methods involve disrupting gene expression but these techniques are only effective against newly synthesized proteins and leave previously existing and stable proteins untouched. Here, we introduce a technique that induces the rapid degradation of specific proteins in mammalian cells by shuttling the proteins to the proteasome for degradation in a ubiquitin-independent manner. We present two implementations of the system in human culture cells that can be used individually to control protein concentration. Our study presents a simple, robust, and flexible technology platform for manipulating intracellular protein levels.