DIRECT MEASUREMENT OF SPECIFIC BINDING OF HIGHLY LIPOPHILIC PHORBOL DIESTER TO MOUSE EPIDERMAL MEMBRANES USING COLD ACETONE
DIRECT MEASUREMENT OF SPECIFIC BINDING OF HIGHLY LIPOPHILIC PHORBOL DIESTER TO MOUSE EPIDERMAL MEMBRANES USING COLD ACETONE
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DOI：
10.1016/0006-291x(81)91779-4
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发表时间：
1981-01-01
影响因子：
3.1
通讯作者：
BOUTWELL, RK
中科院分区：
文献类型：
--
作者：
ASHENDEL, CL;BOUTWELL, RK
用丙酮冷却至-78度。C用[致癌物][3H]12- o -十四烷基磷酸-13-乙酸酯孵育后洗涤小鼠表皮膜制剂，配体的非特异性结合减少100倍，使特异性结合易于定量。该技术可用于其他高脂溶性化学物质与膜成分的结合研究。
Using acetone cooled to -78.degree. C to wash a mouse epidermal membrane preparation after incubation with [the carcinogen] [3H]12-O-tetradecanoylphorbol-13-acetate, nonspecific binding of the ligand is reduced by 100-fold, allowing specific binding to be easily quantitated. This technique may be useful in binding studies of other highly lipid soluble chemicals to membrane constituents.