IMMUNOLOGICAL STUDIES OF THE FUNCTIONS OF PARAMYXOVIRUS GLYCOPROTEINS
IMMUNOLOGICAL STUDIES OF THE FUNCTIONS OF PARAMYXOVIRUS GLYCOPROTEINS
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DOI：
10.1016/0042-6822(81)90474-8
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发表时间：
1981-01-01
期刊：
影响因子：
3.7
通讯作者：
CHOPPIN, PW
中科院分区：
文献类型：
--
作者：
MERZ, DC;SCHEID, A;CHOPPIN, PW
纯化副粘病毒SV 5 [猿猴病毒5型]的HN和F糖蛋白，并制备各自的单特异性抗体。测定这些抗体的二价（IgG）和单价（Fab）形式对糖蛋白的生物活性的影响。抗HN抗体可抑制病毒对红细胞的吸附、血凝和溶血。抑制溶血作用仅次于抑制吸附作用;病毒吸附后加入抗HN抗体对溶血作用无影响。抗HN抗体抑制神经氨酸酶活性，并且在允许病毒和抗体反应并添加不同大小的底物的实验中，这种抑制与底物大小无关，即，神经氨乳糖和胎球蛋白。这一结果与先前流感病毒的发现相反，在先前的流感病毒中，抗体仅抑制病毒酶对大分子底物的作用。因此，对于SV 5，抗体似乎直接抑制水解过程，而不是像流感病毒那样在空间上阻碍酶接近大底物。抗F抗体对病毒吸附或神经氨酸酶活性没有影响，但在吸收前或吸收后加入时抑制溶血，证实F蛋白直接参与溶血过程。通过Fab片段以及IgG实现了通过相应抗体对病毒吸附、神经氨酸酶和溶血活性的抑制，这表明在每种情况下，抑制活性是对单个糖蛋白分子的直接作用的结果，而不是糖蛋白的交联或病毒体的聚集。抗HN和抗F抗体中和了MDBK [Madin-Darby牛肾]和CV-1 [非洲绿色猴肾]细胞中的病毒感染性。抗F IgG和Fab以及抗HN IgG在两种细胞类型中引起基本上完全中和;抗HN Fab在CV-1细胞中仅引起部分中和。
The HN and F glycoproteins of the paramyxovirus SV5 [simian virus type 5] were purified and monospecific antibodies to each prepared. The effects of bivalent (IgG) and monovalent (Fab) forms of these antibodies on the biological activities of the glycoproteins were determined. Anti-HN antibodies inhibited adsorption of virus to erythrocytes, hemagglutination and hemolysis. Inhibition of hemolysis was secondary to the inhibition of adsorption; anti-HN antibodies added after virus adsorption did not affect hemolysis. Anti-HN antibodies inhibited neuraminidase activity and this inhibition was independent of substrate size in experiments in which virus and antibodies were allowed to react and substrates of different size added, i.e., neuraminlactose and fetuin. This result is in contrast to previous findings with influenza virus in which antibodies inhibited the action of the viral enzyme on the macromolecular substrate only. Thus with SV5, the antibodies appear to inhibit the hydrolytic process directly, rather than sterically hindering the access of the enzyme to large substrates, as in the case in influenza virus. Anti-F antibodies had no effect on virus adsorption or neuraminidase activity, but inhibited hemolysis when added before or after absorption, confirming the direct involvement of the F protein in the hemolytic process. The inhibition of virus adsorption, neuraminidase and hemolytic activities by the respective antibodies was accomplished by Fab fragments as well as IgG, indicating that in each case the inhibitory activity was a consequence of a direct effect on individual glycoprotein molecules rather than crosslinking of glycoproteins or aggregation of virions. Anti-HN and anti-F antibodies neutralized virus infectivity in MDBK [Madin-Darby bovine kidney] and CV-1 [African green monkey kidney] cells. Anti-F IgG and Fab and anti-HN IgG caused essentially complete neutralization in both cell types; anti-HN Fab caused only partial neutralization in CV-1 cells.