Self-recognition of N-linked glycans with multivalent GlcNAc, determined as ceramide mimetic conjugate.
Self-recognition of N-linked glycans with multivalent GlcNAc, determined as ceramide mimetic conjugate.
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N-连接聚糖与多价 GlcNAc 的自我识别，确定为神经酰胺模拟缀合物。
DOI：
10.1093/glycob/cwm069
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发表时间：
2007
期刊：
影响因子：
4.3
通讯作者：
Kiso,Makoto
中科院分区：
文献类型：
--
作者：
Yoon,Seon-Joo;Ikeda,Shoko;Sadilek,Martin;Hakomori,Sen-itiroh;Ishida,Hideharu;Kiso,Makoto
合成了氨基乙酰胺模拟物，并通过还原胺化反应将其偶联到含多价GlcNAc的N-连接低聚糖上。神经酰胺与具有五个或六个GlcNAc末端的“复合型”多糖结合(称为Os Fr.B-Cer)，用薄层层析(TLC)进行分析，最后用液相色谱/质谱联用(MS/MS)进行表征。Os Fr.的结合。用含Os Fr的[~3H]胆固醇标记脂质体将B-Cer固定在聚苯乙烯固相表面。B-Cer，或含有各种糖鞘糖脂(GSLS)。Os Fr.的结合。B-Cer脂质体为Os Fr.B-Cer包衣板的结合率显著高于GM3脂质体结合率。其他GSL脂质体未见结合。因此，对OS Fr.的自我认识。用神经酰胺模拟偶联物清楚地显示了B-Cer的存在。
Aminoceramide mimetic was synthesized and conjugated to N-linked oligosaccharides having multivalent GlcNAc by reductive amination. Ceramide mimetic conjugates with “complex-type” glycan having five or six GlcNAc termini (termed Os Fr. B-Cer) were purified, analyzed by thin-layer chromatography (TLC), and finally characterized by MS/MS analysis through liquid chromatography/mass spectrometry. Binding of Os Fr. B-Cer placed on solid phase polystyrene surface with [3H]cholesterol-labeled liposomes containing Os Fr. B-Cer, or containing various glyco- sphingolipids (GSLs) was determined. The binding of Os Fr. B-Cer liposomes to Os Fr. B-Cer coated plate was significantly higher than binding of GM3 liposomes. Other GSL liposomes showed no binding. Thus, self-recognition of Os Fr. B-Cer was clearly demonstrated using ceramide mimetic conjugates.