Complexity of Plasmodium falciparum Clinical Samples from Uganda during Short-Term Culture
Complexity of Plasmodium falciparum Clinical Samples from Uganda during Short-Term Culture
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DOI：
10.1086/592506
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发表时间：
2008-11-15
影响因子：
6.4
通讯作者：
Rosenthal, Philip J.
中科院分区：
文献类型：
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作者：
Nsobya, Samuel L.;Kiggundu, Moses;Rosenthal, Philip J.
我们培养了98名乌干达疟疾儿童的恶性疟原虫寄生虫，并在msp-2多态性的基础上每天测定感染的复杂性（COI），持续9天。培养期间平均COI从1.73降至1.56。在20个培养物中，第0天后出现新菌株。在最初混合的45种培养物中，56%的菌株在第0天后消失;持续存在的菌株通常具有野生型dhfr（C59）和dhps（K540）序列以及突变型pfmdr 1（86 Y）序列。因此，初始基因型不能完全代表临床COI。培养物中菌株的丢失可能是由于某些耐药菌株的适应性降低。
We cultured Plasmodium falciparum parasites from 98 Ugandan children with malaria and determined the complexity of infection (COI) on the basis of msp-2 polymorphisms daily for 9 days. The mean COI decreased during culture from 1.73 to 1.56. New strains appeared after day 0 in 20 cultures. Strains disappeared after day 0 in 56% of 45 cultures that were initially mixed; persisting strains more commonly had wild-type dhfr (C59) and dhps (K540) sequences and mutant pfmdr1 (86Y) sequences. Thus, initial genotypes offer an imperfect representation of clinical COI. Loss of strains in culture may be due to diminished fitness of some drug-resistant strains.