Sequence-tagged-site (STS) markers of arbitrary genes: development, characterization and analysis of linkage in black spruce.
Sequence-tagged-site (STS) markers of arbitrary genes: development, characterization and analysis of linkage in black spruce.
复制标题
任意基因的序列标记位点（STS）标记：黑云杉连锁的发育、表征和分析。
作者：
D. J. Perry;J. Bousquet
对黑云杉（Picea mariana (Mill.)）中任意基因的序列标记位点（STS）标记进行了研究。B.S.P.]。39对PCR引物用于筛选单倍体和二倍体dna的不同片段，以便在不进一步操作扩增产物的情况下通过标准琼脂糖凝胶电泳检测到变异。共显性长度多态性分布在15个位点。其中3个基因座也有零扩增等位基因，另外3个基因座也没有明显的产物长度变异。另外2个位点存在显性长度多态性。共显性标记的等位基因大小差异小至1 bp，大至175 bp，几乎所有的插入/缺失都在非编码区发现。3个位点的多态性涉及大的（33 bp至至少114 bp）直接重复，51个cdna序列中有7个发现类似重复。等位基因分离符合孟德尔遗传，63个基因座成对组合中有5个存在连锁。共显性STS标记的12个位点的平均杂合度为0.26，等位基因的平均杂合度为2.8。
Sequence-tagged-site (STS) markers of arbitrary genes were investigated in black spruce [Picea mariana (Mill.) B.S.P.]. Thirty-nine pairs of PCR primers were used to screen diverse panels of haploid and diploid DNAs for variation that could be detected by standard agarose gel electrophoresis without further manipulation of amplification products. Codominant length polymorphisms were revealed at 15 loci. Three of these loci also had null amplification alleles as did 3 other loci that had no apparent product-length variation. Dominant length polymorphisms were observed at 2 other loci. Alleles of codominant markers differed in size by as little as 1 bp to as much as an estimated 175 bp with nearly all insertions/deletions found in noncoding regions. Polymorphisms at 3 loci involved large (33 bp to at least 114 bp) direct repeats and similar repeats were found in 7 of 51 cDNAs sequenced. Allelic segregation was in accordance with Mendelian inheritance and linkage was detected for 5 of 63 pairwise combinations of loci tested. Codominant STS markers of 12 loci revealed an average heterozygosity of 0.26 and an average of 2.8 alleles in a range-wide sample of 22 trees.