A subpopulation of CD163-positive macrophages is classically activated in psoriasis.
A subpopulation of CD163-positive macrophages is classically activated in psoriasis.
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DOI：
10.1038/jid.2010.165
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发表时间：
2010-10
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影响因子：
--
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中科院分区：
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巨噬细胞是天然免疫系统的重要细胞，对巨噬细胞的研究对于更好地了解银屑病的炎症本质是必不可少的。我们用免疫组织化学和双标记免疫荧光技术对银屑病患者的CD163+巨噬细胞进行了研究。与正常皮肤相比，银屑病患者真皮巨噬细胞增多，CD163、RFD7、CD68、LAMP2、Stablin-1和Marco鉴定。CD163+巨噬细胞表达C-凝集素CD206/MMR和CD209/DC-SIGN，以及共刺激分子CD86和CD40。不表达成熟的DC标志物CD208/DC-LAMP、CD205/DEC205或CD83。对体外培养的巨噬细胞经干扰素γ处理后的基因芯片分析表明，许多巨噬细胞上调的基因在银屑病中被发现，包括STAT1、CXCL9、MX1和HLADR。CD163+巨噬细胞产生炎症分子IL-23p19和IL-12/23p40以及肿瘤坏死因子和诱导型一氧化氮合酶。这些数据表明，CD163是巨噬细胞的一个较好的标记物，并确定了银屑病中一种“经典激活的”巨噬细胞亚群。我们得出结论，巨噬细胞可能通过释放关键的炎症产物，在银屑病的病理性炎症中做出贡献，银屑病是一种典型的Th1和Th17疾病。
Macrophages are important cells of the innate immune system, and their study is essential to gain greater understanding of the inflammatory nature of psoriasis. We used immunohistochemistry and double-label immunofluorescence to characterize CD163+ macrophages in psoriasis. Dermal macrophages were increased in psoriasis compared to normal skin and were identified by CD163, RFD7, CD68, LAMP2, Stabilin-1, and MARCO. CD163+ macrophages expressed C-lectins CD206/MMR and CD209/DC-SIGN, as well as co-stimulatory molecules CD86 and CD40. They did not express mature DC markers CD208/DC-LAMP, CD205/DEC205 or CD83. Microarray analysis of in vitro derived macrophages treated with IFNγ showed that many of the genes upregulated in macrophages were found in psoriasis, including STAT1, CXCL9, Mx1 and HLA-DR. CD163+ macrophages produced inflammatory molecules IL-23p19 and IL-12/23p40 as well as TNF and iNOS. These data demonstrate that CD163 is a superior marker of macrophages, and identifies a subpopulation of “classically activated” macrophages in psoriasis. We conclude that macrophages are likely to be contributing to the pathogenic inflammation in psoriasis, a prototypical Th1 and Th17 disease, by releasing key inflammatory products.
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DOI：
10.4049/jimmunol.181.7.4733
发表时间：
2008-10-01
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Kryczek I;Bruce AT;Gudjonsson JE;Johnston A;Aphale A;Vatan L;Szeliga W;Wang Y;Liu Y;Welling TH;Elder JT;Zou W
通讯作者：
Zou W
DOI：
10.1084/jem.20091683
发表时间：
2009-08-31
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Casanova JL;Abel L
通讯作者：
Abel L
影响因子：
4.4
作者：
Martinez, Fernando O.;Gordon, Siamon;Mantovani, Alberto
通讯作者：
Mantovani, Alberto
影响因子：
3.7
作者：
Gratchev, A;Kzhyshkowska, J;Goerdt, S
通讯作者：
Goerdt, S
影响因子：
5.5
作者：
Kzhyshkowska, J;Gratchev, A;Goerdt, S
通讯作者：
Goerdt, S