Characteristics of Streptolysin O Hemolysis: Kinetics of Hemoglobin and 86Rubidium Release
Characteristics of Streptolysin O Hemolysis: Kinetics of Hemoglobin and 86Rubidium Release
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链球菌溶血素 O 溶血的特征：血红蛋白和 86 铷释放的动力学
DOI：
10.1128/iai.9.6.1022-1027.1974
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发表时间：
1974
影响因子：
3.1
通讯作者：
J. Duncan
中科院分区：
文献类型：
--
作者：
J. Duncan
研究了链球菌溶血素O（SLO）对家兔、人和大鼠红细胞的溶血特性。血红蛋白（Hb）释放的动力学研究表明，兔和人红细胞呈现典型的“多击”存活曲线。将这些曲线外推到纵坐标表明，大约两个分子的SLO可能足以产生单个细胞的裂解。相反，当用SLO处理大鼠红细胞时，观察到指数（“单次击中”）存活曲线。在0 ℃时，高浓度的SLO迅速溶解兔红细胞;低浓度的SLO在此温度下对细胞没有影响。本文研究了SLO对家兔红细胞内~（86）Rb ~+和Hb释放的影响。在磷酸盐缓冲盐水的存在下，铷和血红蛋白从毒素处理的细胞中以相同的速率丢失。加入牛血清白蛋白的悬浮液中并没有阻止逃逸的Hb，和流出的86 Rb+只有轻微的Hb损失之前。添加蔗糖的细胞延迟血红蛋白和铷的释放。这些结果被解释为表明“胶体渗透”溶解过程不参与链球菌溶血素O对红细胞的溶血。
The characteristics of hemolysis produced by streptolysin O (SLO) were investigated in rabbit, human, and rat erythrocytes. Kinetic studies of hemoglobin (Hb) release showed that rabbit and human erythrocytes exhibited typical “multi-hit” survival curves. Extrapolation of these curves to the ordinate indicated that approximately two molecules of SLO may be sufficient to produce lysis of a single cell. In contrast, exponential (“single-hit”) survival curves were observed when rat erythrocytes were treated with SLO. At 0 C, high concentrations of SLO rapidly lysed rabbit erythrocytes; low concentrations had no effect on the cells at this temperature. The release of intracellular 86rubidium (86Rb+) and Hb in rabbit erythrocytes exposed to SLO was investigated. In the presence of phosphate-buffered saline, rubidium and Hb were lost at the same rate from toxin-treated cells. The addition of bovine serum albumin to the suspending medium did not retard the escape of Hb, and the efflux of 86Rb+ only slightly preceded Hb loss. Addition of sucrose to the cells delayed both Hb and rubidium release. These results are interpreted as indicating that the “colloid-osmotic” lytic process is not involved in the hemolysis of erythrocytes by streptolysin O.