MiR-932 Regulates Pyrethroid Resistance in Culex pipiens pallens (Diptera: Culicidae)
MiR-932 Regulates Pyrethroid Resistance in Culex pipiens pallens (Diptera: Culicidae)
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MiR-932 调节淡色库蚊（双翅目：库蚊科）对拟除虫菊酯的抗性
DOI：
10.1093/jme/tjw083
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发表时间：
2016-09-01
影响因子：
2.1
通讯作者：
Zhu, Changliang
中科院分区：
文献类型：
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作者：
Liu, Bingqian;Tian, Mengmeng;Zhu, Changliang
摘要微小RNA（miRNAs）在转录后水平对基因表达起着重要的调控作用，并参与了包括杀虫剂抗性在内的许多生物学过程。在这项研究中，我们研究了miR-932在淡色库蚊对拟除虫菊酯抗性的分子机制中的作用。在DS品系中过表达miR-932使蚊子对溴氰菊酯更具抗性，而在DR品系中抑制miR-932的表达使蚊子对溴氰菊酯更敏感。此外，我们还鉴定了CpCPR 5作为miR-932的靶基因。miR-932的持续过表达导致CpCPR 5的抑制，并且miR-932的敲低增加CpCPR 5的表达。此外，CpCPR 5的敲低降低了DS品系蚊子对溴氰菊酯的敏感性。总之，我们的研究发现了miR-932和CpCPR 5之间的分子联系，并为杀虫剂抗性机制提供了新的见解。
Abstract MicroRNAs (miRNAs) play notable role in regulation of gene expression at the posttranscription level, and have been involved in many biological processes, including insecticide resistance. In this study, we investigated the role of miR-932 in the molecular mechanisms of pyrethroid resistance in Culex pipiens pallens (L.). Overexpression of miR-932 in the DS-strain made the mosquitoes more resistant to deltamethrin, while inhibiting the expression of miR-932 in the DR-strain made the mosquitoes more sensitive to deltamethrin. Further, we also identified CpCPR5 as a target gene of miR-932. Sustained overexpression of miR-932 resulted in repression of CpCPR5, and that knockdown of miR-932 increased CpCPR5 expression. In addition, knockdown of CpCPR5 decreased the sensitivity of mosquitoes to deltamethrin in the DS-strain. In conclusion, our study finds a molecular link between miR-932 and CpCPR5 and provides a novel insight into the mechanism of insecticide resistance.