Virus persists in beta cells of islets of Langerhans and is associated with chemical manifestations of diabetes.
Virus persists in beta cells of islets of Langerhans and is associated with chemical manifestations of diabetes.
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DOI：
10.1126/science.6203172
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发表时间：
1984-06
期刊：
影响因子：
56.9
通讯作者：
M. B. Oldstone;P. J. Southern;M. Rodríquez;P. W. Lampert
中科院分区：
文献类型：
--
作者：
M. B. Oldstone;P. J. Southern;M. Rodríquez;P. W. Lampert
分子杂交、单克隆抗体和电镜分析显示，淋巴细胞性脉络丛脑膜炎病毒（株Armstrong和株WE）持续感染BALB/WEHI小鼠朗格汉斯岛细胞。用两种不同的荧光染料偶联的单克隆或单特异性抗体标记含胰岛素的β细胞或病毒抗原时，主要在β细胞中鉴定出病毒核蛋白。电子显微镜通过显示病毒粒子从细胞出芽证实了这些发现。持续感染与糖尿病的化学证据相关（高血糖、糖耐量异常、胰岛素浓度正常或低正常）。血液中皮质醇和胰岛素样生长因子的浓度正常，脑垂体中生长激素的水平也正常。病毒感染的胰岛细胞解剖和细胞形态正常。常规未见细胞溶解或炎症浸润。因此，一种病毒可能持续感染胰岛细胞，并提供与人类早期成人发病糖尿病相似的生化和形态学图像。
Molecular hybridization, monoclonal antibody, and electron microscopic analyses showed lymphocytic choriomeningitis virus (strains Armstrong and WE) persistently infecting cells of the islets of Langerhans in BALB/WEHI mice. When monoclonal or monospecific antibody conjugated with two different fluorochrome dyes was used to mark insulin-containing beta cells or viral antigens, viral nucleoprotein was identified predominantly in beta cells. Electron microscopy confirmed these findings by showing virions budding from the beta cells. Persistent infection was associated with chemical evidence of diabetes (hyperglycemia, abnormal glucose tolerance, and normal or low-normal concentrations of insulin). Concentrations of cortisol and insulin-like growth factor in blood were normal, as was the level of growth hormone in the pituitary gland. The virus-infected islet cells showed normal anatomy and cytomorphology. Neither cell lysis nor inflammatory infiltrates were routinely seen. Thus a virus may persistently infect islet cells and provide a biochemical and morphological picture comparable to that of early adult-onset diabetes mellitus in humans.