Plasmodium gallinaceum:: Differential killing of some mosquito stages of the parasite by insect defensin
Plasmodium gallinaceum:: Differential killing of some mosquito stages of the parasite by insect defensin
复制标题
DOI：
10.1006/expr.1998.4212
复制
发表时间：
1998-05-01
影响因子：
2.1
通讯作者：
Miller, LH
中科院分区：
文献类型：
--
作者：
Shahabuddin, M;Fields, I;Miller, LH
我们检测了几种昆虫抗菌肽，以研究它们对鸡疟原虫受精卵、动合子、卵囊和子孢子的影响。只有两种昆虫防御素--蓝纹伊蚊（蜻蜓）和地平蝇（肉蝇）--对埃及伊蚊的卵囊和分离的子孢子具有严重的毒性作用。防御素对卵囊的影响呈时间依赖性。在感染性血餐后1或2天将肽注射到血淋巴中对感染的流行率或每只蚊子的相对卵囊密度没有显著影响。当注射寄生虫摄入后3天，相对卵囊密度显着降低。在第4天或之后注射破坏了发育中的卵囊，尽管在第8天检查时，每只蚊子的卵囊密度没有显著差异。卵囊肿胀或有广泛的内部空泡化。这些肽对寄生虫的早期阶段没有可检测到的影响：体外测试的受精卵和动合子。这两种防御素在体外对分离的子孢子具有高毒性，如通过破坏膜渗透屏障、形态变化和丧失运动性所示。与抗菌肽和爪蟾抗菌肽对蚊子的毒性相反，在杀死寄生虫的浓度下，防御素对蚊子没有毒性，这表明防御素作为一种潜在的分子应进一步研究，以阻止疟原虫的孢子生殖发育。(C)北京：科学出版社.
We examined several insect antimicrobial peptides to study their effect on Plasmodium gallinaceum zygotes, ookinetes, oocysts, and sporozoites. Only two insect defensins-Aeschna cyanea (dragon fly) and Phormia terranovae (flesh fly)-had a profound toxic effect on the oocysts in Aedes aegypti and on isolated sporozoites. The defensins affected the oocysts in a time-dependent manner. Injecting the peptide into the hemolymph 1 or 2 days after an infectious blood meal had no significant effect on prevalence of infection or relative oocyst density per mosquito. When injected 3 days after parasite ingestion, the relative oocyst density was significantly reduced. Injection on day 4 or later damaged the developing oocysts, although the oocysts density per mosquito was not significantly different when examined on day 8. The oocysts were swollen or had extensive internal vacuolization. The peptides had no detectable effect on the early stages of the parasite: the zygotes and ookinetes tested in vitro. Both the defensins were highly toxic to isolated sporozoites in vitro as indicated by disruption of the membrane permeability barrier, a change in morphology, and loss of motility. In contrast to the toxicity of cecropin and magainin for mosquitoes, defensin, at concentrations that kill parasites, is not toxic to mosquitoes, suggesting that defensin should be studied further as a potential molecule to block sporogonic development of Plasmodium. (C) 1998 Academic Press.