Direct antagonistic effect of entomopathogenic nematodes and their symbiotic bacteria on root-knot nematodes migration toward tomato roots
Direct antagonistic effect of entomopathogenic nematodes and their symbiotic bacteria on root-knot nematodes migration toward tomato roots
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DOI：
10.1007/s11104-022-05808-4
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发表时间：
2022-12
期刊：
影响因子：
4.9
通讯作者：
Jingjing Li;Yang Li;Xianqin Wei;Yonghe Cui;X. Gu;Xingyue Li;T. Yoshiga;M. Abd-Elgawad;D. Shapiro-Ilan;W. Ruan;S. Rasmann
中科院分区：
文献类型：
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作者：
Jingjing Li;Yang Li;Xianqin Wei;Yonghe Cui;X. Gu;Xingyue Li;T. Yoshiga;M. Abd-Elgawad;D. Shapiro-Ilan;W. Ruan;S. Rasmann
目的在过去的20年里，根结线虫等植物寄生线虫与昆虫病原线虫(EPN)在根际的负相互作用已有报道，但这种相互作用的机制和动态仍很难理解。方法我们评价了两种EPN的接种位置以及EPN-细菌复合体的不同方面对RKN向番茄根部迁移的影响。结果当EPN放置在根结线虫与根之间时，RKN向根的移动被抑制。我们在沙子和Pluronic F-127(PF-127)凝胶中观察到了两种EPN的相同模式。我们还观察到EPNS/细菌共生体的不同成分(从线虫和线虫-细菌复合体中分离出来的细菌)，特别是细菌培养产生的无细胞上清液，对RKN具有抑制作用。结论EPNS/细菌复合体通过减缓RKN向寄主植物根部的移动，可能在一定程度上有助于RKN的控制。通过筛选对害虫也有效的EPN最令人厌恶的菌株，综合靶标方法应该会减少EPN在综合害虫管理实践中的应用成本。
AimsNegative interactions in the rhizosphere between entomopathogenic nematodes (EPNs) and plant-parasitic nematodes, such as root-knot nematodes (RKNs), have been documented over the past two decades but the mechanisms and dynamics of such interactions remain largely elusive.MethodsHere, we evaluated the effect of the inoculation position of two EPN species,Steinernema feltiaeandHeterorhabditis bacteriophora, as well as different facets of the EPN-bacterial symbiont complex on the migration of RKNs toward tomato roots, both in sand and in Pluronic gel conditions.ResultsWhen EPNs were placed between the position of the RKNs and the roots, the movement of RKNs toward the roots was inhibited. We observed this same pattern both in sand and in Pluronic F-127 (PF-127) gel for two species of EPNs. We also observed that different components of the EPNs/bacterial symbiont complex (bacteria separate from the nematodes vs. the nematode-bacterium complex), and particularly the cell-free supernatant produced by the bacterial culture, displayed inhibitory effects on RKNs.ConclusionTherefore, the EPNs/bacterial complex, by slowing down the movement of RKNs toward the host plant roots, could partially contribute to RKN control. By screening for the most repulsive strains of EPNs that are also effective against insect pests, the combined target approach should alleviate EPNs application costs in integrated pest management practices.