HSV-1 in brain and risk of Alzheimer's disease
HSV-1 in brain and risk of Alzheimer's disease
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DOI：
10.1016/s0140-6736(05)79057-7
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发表时间：
1998-05-02
期刊：
影响因子：
168.9
通讯作者：
Poirier, J
中科院分区：
文献类型：
--
作者：
Beffert, U;Bertrand, P;Poirier, J
研究了73名患有神经病理学证实的阿尔茨海默病的老年人（34名男性，年龄范围53-93岁）和33名无阿尔茨海默病的老年人（27名男性，年龄范围43-95岁）的大脑[未给出日期和地点]。从脑组织中提取DNA，并用作模板，通过聚合酶链反应，扩增来自单纯疱疹病毒1型（HSV-1）糖蛋白D基因的138 bp片段。用等位基因特异性引物检测载脂蛋白E（apoE）基因型。单纯疱疹病毒1型（HSV-1）阳性的阿尔茨海默病患者与单纯疱疹病毒1型（HSV-1）阴性的阿尔茨海默病患者的apoE ε4等位基因频率差异无统计学意义（37 vs.24%，P =0.19，经χ2检验）。HSV-1阳性率在阿尔茨海默病患者和对照组之间没有显著差异（74 vs. 73%;P=0.89）。与非ε4等位基因携带者相比，在不考虑病理的情况下，未发现apoE ε4等位基因携带者对HSV-1感染的易感性增加（81 vs. 68%;P=0.20）。得出的结论是，当与apoE的ε4等位基因的存在结合时，单纯疱疹病毒-1并不会增加阿尔茨海默病的风险，单纯疱疹病毒-1本身并不是阿尔茨海默病的独立危险因素，并且apoE ε4等位基因携带者并不更容易感染。比非携带者更容易感染。
Brains from 73 elderly individuals with neuropathologically-confirmed Alzheimer's disease (34 men, age range 53-93 years) and 33 without Alzheimer's disease (27 men, age range 43-95 years) were investigated [date and place not given]. DNA was extracted from brain tissue and used as a template to amplify, by polymerase chain reaction, a 138 bp fragment from the glycoprotein D gene of herpes simplex virus 1 (HSV-1). Apolipoprotein E (apoE) genotype was determined with allele-specific primers. There was no significant difference in the observed apoE ε4 allele frequency between HSV-1-positive Alzheimer's disease patients and HSV-1-negative patients (37 vs. 24%,P=0.19 by χ2). HSV-1-positive ratio did not differ significantly between Alzheimer's disease patients and controls (74 vs. 73%;P=0.89). No increased susceptibility to HSV-1 infection was found among apoE ε4 allele carriers compared with non-ε4 carriers irrespective of pathology (81 vs. 68%;P=0.20). It is concluded that HSV-1 does not confer increased risk for Alzheimer's disease when combined with the presence of an ε4 allele of apoE, that HSV-1 alone is not an independent risk factor for Alzheimer's disease and that apoE ε4 allele carriers are not more susceptible to HSV-1 infection than non-carriers.