Endothelial cells grown on thin polyelectrolyte mutlilayered films: an evaluation of a new versatile surface modification
Endothelial cells grown on thin polyelectrolyte mutlilayered films: an evaluation of a new versatile surface modification
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DOI：
10.1016/s0142-9612(03)00214-x
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发表时间：
2003-09-01
期刊：
影响因子：
14
通讯作者：
Stoltz, JF
中科院分区：
文献类型：
--
作者：
Boura, C;Menu, P;Stoltz, JF
内皮细胞接种是改善小直径血管移植物血液相容性的一种值得赞赏的方法。在这项研究中，我们报道了一种基于多层聚电解质膜作为细胞黏附基板的简单创新技术的发展。以poly(sodium-4-styrenesulfonate)/poly(allylamine盐酸盐(PSS/PAH)或L-谷氨酸/聚D-赖氨酸(PGA/PDL)封端的聚电解质多层膜可以通过修饰表面的物理化学性质来增强细胞的粘附性。将人脐静脉内皮细胞接种于聚电解质多层膜、PDL或PAH单层和对照表面，从初始附着、生长、细胞代谢活性、内皮表型和黏附等方面评价其生物学反应。结果表明，聚电解质多层膜不会引起细胞毒作用，也不会改变内皮细胞的表型。与聚电解质单层相比，聚电解质多层膜增强了电池的初始附着。在薄膜上观察到的细胞生长与在TCPS上观察到的相似。在所测试的不同涂层中，以PSS/PAH结束的涂层表现出良好的细胞生物相容性，并且在细胞黏附和生长方面似乎是最有趣的表面。这种薄膜可用于覆盖疏水性(抗细胞)底物，以促进细胞定植，从而构成一种良好的内皮细胞种植材料。(C)2003爱思唯尔科学有限公司。保留所有权利。
Endothelial cell seeding constitutes an appreciated method to improve blood compatibility of small-diameter vascular grafts. In this study, we report the development of a simple innovative technique based on multilayered polyelectrolyte films as cell adhesive substrates. Polyelectrolyte multilayered films ending by poly(sodium-4-styrenesulfonate)/poly(allylamine hydrochloride) (PSS/PAH) or poly(L-glutamic acid)/poly(D-lysine) (PGA/PDL) could enhance cell adhesion by modification of the physico-chemical properties of the surface. The biological responses of human umbilical vein endothelial cells seeded on the polyelectrolyte multilayer films, on PDL or PAH monolayers, and on control surfaces, were evaluated in terms of initial attachment, growth, cellular metabolic activity, endothelial phenotype, and adhesion. The results showed that polyelectrolyte multilayers neither induce cytotoxic effects nor alter the phenotype of the endothelial cells. The polyelectrolyte multilayered films enhanced initial cell attachment as compared to the polyelectrolyte monolayer. Cell growth observed on the films was similar to that on TCPS. Among the different coating tested, the film ending by PSS/PAH exhibited an excellent cellular biocompatibility and appeared to be the most interesting surface in terms of cellular adhesion and growth. Such films could be used to cover hydrophobic (cell resistant) substrates in order to promote cell colonization, thereby constituting an excellent material for endothelial cell seeding. (C) 2003 Elsevier Science Ltd. All rights reserved.