MHC class II and invariant chain biosynthesis and transport during maturation of human precursor dendritic cells
MHC class II and invariant chain biosynthesis and transport during maturation of human precursor dendritic cells
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DOI：
10.1093/intimm/10.11.1713
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发表时间：
1998-11-01
影响因子：
4.4
通讯作者：
Pieters, J
中科院分区：
文献类型：
--
作者：
Engering, AJ;Richters, CD;Pieters, J
树突状细胞（DC）是免疫应答的高效激活剂。产生DC表型的确切机制尚不清楚。为了研究有助于产生DC表型的机制，从人血液中新鲜分离前体DC并使其在体外成熟。这些成熟的DC表现出DC的表型和功能特征。MHC II类和不变链（li）的生物合成的分析表明，成熟后，II类合成诱导，而ii合成显着上调。在成熟的DC中，尽管存在大量的ii，出口的MHC II类分子从内质网是不完整的，生物合成后4小时。因此，DC中的MHC II-ii类合成和转运在DC成熟期间受到高度调节。分析其调控机制有助于更好地理解DC分化过程中的抗原提呈能力。
Dendritic cells (DC) are highly potent activators of the immune response. The precise mechanisms that give rise to the DC phenotype are not known. To investigate the mechanisms that contribute to the generation of the DC phenotype, precursor DC were freshly isolated from human blood and allowed to mature in vitro. These matured DC showed the phenotypical and functional characteristics of DC. Analysis of the MHC class II and invariant chain (li) biosynthesis revealed that upon maturation, class II synthesis was induced whereas ii synthesis was significantly up-regulated. In mature DC, despite the presence of large amounts of ii, export of MHC class II molecules from the endoplasmic reticulum was incomplete, up to 4 h after biosynthesis. Thus, MHC class II-ii synthesis and transport in DC is highly regulated during maturation of DC. Analysis of the regulatory mechanisms may contribute to a better understanding of antigen-presenting capacities during the differentiation of DC.