Identified trans-splicing of YELLOW-FRUITED TOMATO 2 encoding the PHYTOENE SYNTHASE 1 protein alters fruit color by map-based cloning, functional complementation and RACE
Identified trans-splicing of YELLOW-FRUITED TOMATO 2 encoding the PHYTOENE SYNTHASE 1 protein alters fruit color by map-based cloning, functional complementation and RACE
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编码 PHYTOENE SYNTHASE 1 蛋白的黄果番茄 2 的反式剪接通过基于图谱的克隆、功能互补和 RACE 改变了果实颜色
DOI：
10.1007/s11103-019-00886-y
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发表时间：
2019-08-01
影响因子：
5.1
通讯作者：
Zhao, Lingxia
中科院分区：
文献类型：
--
作者：
Chen, Lulu;Li, Wenzhen;Zhao, Lingxia
通过基于图谱的克隆、功能互补和RACE，发现了PHYTOENE合成酶1的反式剪接改变了番茄的果实颜色，为深入了解水果的颜色发育提供了理论基础。颜色是番茄重要的果实品质性状，也是决定番茄经济价值的主要因素。黄果樱桃番茄果实颜色的遗传(cv。No.2)，命名为黄果番茄2号(Yft2)，受1对隐性基因YFT2控制。将YFT2基因定位于3号染色体上95.7kb的区域，功能互补分析确定了候选基因PSY1。与红果番茄品种SL1995和栽培品种(cv.)相比，yft2中PSY1的结构性过表达增加了类胡萝卜素的积累，导致果实颜色变红，而YFT2等位基因没有检测到因果突变。M82)。YFT2中YFT2 3‘区的表达显著低于SL1995，进一步的研究表明YFT2的转录后加工过程与SL1995和cv不同。M82，导致YFT2转录本更长。YFT2中YFT2的选择性反式剪接等位基因被预测编码一个新的LT-YFT2蛋白，该蛋白由432个氨基酸(AA)残基组成，而SL1995的412个氨基酸残基为YFT2蛋白。反式剪接事件还导致YFT2在番茄中的表达显著下调，YFT2等位基因通过前馈调控机制抑制了参与类胡萝卜素生物合成途径和类胡萝卜素合成的下游基因的表达。综上所述，我们发现YFT2的反式剪接改变了番茄果实的颜色，为果实颜色的发育提供了新的见解。
Found a trans-splicing of PHYTOENE SYNTHASE 1 alters tomato fruit color by map-based cloning, functional complementation and RACE providing an insight into fruit color development. Color is an important fruit quality trait and a major determinant of the economic value of tomato (Solanum lycopersicum). Fruit color inheritance in a yellow-fruited cherry tomato (cv. No. 22), named yellow-fruited tomato 2 (yft2), was shown to be controlled by a single recessive gene, YFT2. The YFT2 gene was mapped in a 95.7 kb region on chromosome 3, and the candidate gene, PHYTOENE SYNTHASE 1 (PSY1), was confirmed by functional complementation analysis. Constitutive over expression of PSY1 in yft2 increased the accumulation of carotenoids and resulted in a red fruit color, while no causal mutation was detected in the YFT2 allele of yft2, compared with red-fruited SL1995 cherry tomato or cultivated variety (cv. M82). Expression of YFT2 3′ region in yft2 was significantly lower than in SL1995, and further studies revealed a difference in YFT2 post-transcriptional processing in yft2 compared with SL1995 and cv. M82, resulting in a longer YFT2 transcript. The alternatively trans-spliced allele of YFT2 in yft2 is predicted to encode a novel LT-YFT2 protein of 432 amino acid (AA) residues, compared to the 412 AA YFT2 protein of SL1995. The trans-spliced event also resulted in significantly down regulated expression of YFT2 in yft2 tomato, and the YFT2 allele suppressed expression of the downstream genes involved in the carotenoid biosynthesis pathway and carotenoids synthesis by a mechanism of the feed-forward regulation. In conclusion, we found that trans-splicing of YFT2 alters tomato fruit color, providing new insights into fruit color development.