Lab-on-chip systems for integrated bioanalyses.
Lab-on-chip systems for integrated bioanalyses.
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DOI：
10.1042/ebc20150013
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发表时间：
2016-06-30
影响因子：
6.4
通讯作者：
Chu V
中科院分区：
文献类型：
--
作者：
Conde JP;Madaboosi N;Soares RR;Fernandes JT;Novo P;Moulas G;Chu V
基于微流体的生物分子检测系统通常被称为芯片实验室系统。为了充分受益于微流体带来的小型化，人们的目标是开发“从样品到答案”的分析系统，其中输入是原始或最低限度加工的生物，食品/饲料或环境样品，输出是对一种或多种感兴趣的分析物的定量或定性评估。一般来说，这样的系统将需要整合几个步骤或操作来执行其功能。本综述将讨论这些操作阶段，包括流体处理，它确保所需的流体在适当的时间和适当的流动条件下到达特定的位置；分子识别，允许在芯片上的精确位置捕获特定分析物；分子识别事件转导成可测量信号；分析物捕获上游的样品制备；和信号放大程序，以提高灵敏度。需要不同阶段的无缝集成，以实现护理点/使用点实验室芯片设备，允许在相关灵敏度范围内进行分析物检测，具有竞争力的分析时间和成本。
Biomolecular detection systems based on microfluidics are often called lab-on-chip systems. To fully benefit from the miniaturization resulting from microfluidics, one aims to develop ‘from sample-to-answer’ analytical systems, in which the input is a raw or minimally processed biological, food/feed or environmental sample and the output is a quantitative or qualitative assessment of one or more analytes of interest. In general, such systems will require the integration of several steps or operations to perform their function. This review will discuss these stages of operation, including fluidic handling, which assures that the desired fluid arrives at a specific location at the right time and under the appropriate flow conditions; molecular recognition, which allows the capture of specific analytes at precise locations on the chip; transduction of the molecular recognition event into a measurable signal; sample preparation upstream from analyte capture; and signal amplification procedures to increase sensitivity. Seamless integration of the different stages is required to achieve a point-of-care/point-of-use lab-on-chip device that allows analyte detection at the relevant sensitivity ranges, with a competitive analysis time and cost.