Derivation of microglia-free astrocyte cultures from neural stem cells.
Derivation of microglia-free astrocyte cultures from neural stem cells.
复制标题
从神经干细胞中衍生出无小胶质细胞的星形胶质细胞培养物。
DOI：
10.1007/978-1-61779-452-0_6
复制
发表时间：
2012
期刊：
影响因子：
--
通讯作者：
Milner,Richard
中科院分区：
文献类型：
--
作者：
Welser,JenniferV;Milner,Richard
星形胶质细胞在中枢神经系统（CNS）组织稳态的建立和维持中发挥着重要作用。为了在体外检查这些不同的功能，能够产生纯星形胶质细胞培养物非常重要。虽然已经描述了许多“富集”星形胶质细胞培养物，但由于其他污染细胞类型（例如小胶质细胞）的存在，这些培养物变得复杂。在本章中，我们描述了一种从神经球产生无小胶质细胞的星形胶质细胞培养物的方法，并且还包括免疫细胞化学方法来证明这些培养物的纯度。
Astrocytes play fundamental roles in the establishment and maintenance of tissue homeostasis in the central nervous system (CNS). To examine these different functions in vitro, it is important to be able to generate pure astrocyte cultures. While many “enriched” astrocyte cultures have been described, these are complicated by the presence of other contaminating cell types, such as microglia. In this chapter, we describe a method in which microglia-free astrocyte cultures are generated from neurospheres and also include an immunocytochemical approach to demonstrate the purity of these cultures.