Members of the miR-30 family inhibit the epithelial-to-mesenchymal transition of non-small-cell lung cancer cells by suppressing XB130 expression levels
Members of the miR-30 family inhibit the epithelial-to-mesenchymal transition of non-small-cell lung cancer cells by suppressing XB130 expression levels
复制标题
miR-30家族成员通过抑制XB130表达水平来抑制非小细胞肺癌细胞的上皮间质转化
DOI：
10.3892/ol.2020.11929
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发表时间：
2020-10-01
期刊：
影响因子：
2.9
通讯作者：
Wang, Qinrong
中科院分区：
文献类型：
--
作者：
Song, Kewei;Jiang, Yinhui;Wang, Qinrong
microRNA（miRs）与癌症转移相关。在非小细胞肺癌（NSCLC）中观察到miR-30家族成员的异常表达水平。然而，miR-30家族成员对NSCLC细胞上皮间质转化（EMT）的影响及其潜在的分子机制尚未完全阐明。本研究探讨miR-30家族成员对NSCLC细胞EMT、迁移和侵袭的影响，发现miR-30过表达可通过降低N-cadherin、β-catenin和SNAI 1的表达水平抑制EMT，沿着迁移和侵袭能力的减弱。然后，将XB 130鉴定为miR-30家族成员的下游靶标。XB 130敲除也抑制了NSCLC细胞的EMT，而XB 130的异位过表达部分挽救了miR-30 c和miR-30 d对EMT的抑制作用。总之，miR-30家族成员部分通过抑制XB 130表达水平来抑制NSCLC细胞的EMT。
MicroRNAs (miRs) are associated with cancer metastasis. Aberrant expression levels of members of the miR-30 family have been observed in non-small-cell lung cancer (NSCLC). However, the effects of miR-30 family members on the epithelial-to-mesenchymal transition (EMT) of NSCLC cells and the underlying molecular mechanisms have not yet been fully elucidated. The present study investigated the effects of miR-30 family members on EMT, migration and invasion of NSCLC cells and found that overexpression of these miRs inhibited EMT via decreasing the expression levels of N-cadherin, β-catenin and SNAI1, along with weakened migration and invasion abilities. Then, XB130 was identified as a downstream target of the miR-30 family members. XB130-knockdown also inhibited EMT of NSCLC cells, whereas ectopic overexpression of XB130 partly rescued the suppressive effects of miR-30c and miR-30d on EMT. In conclusion, miR-30 family members inhibited EMT of NSCLC cells, partially via suppressing XB130 expression levels.