Unconventional conjugal DNA transfer in mycobacteria.
Unconventional conjugal DNA transfer in mycobacteria.
复制标题
分枝杆菌中的非常规配偶 DNA 转移。
DOI：
10.1038/ng1139
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发表时间：
2003
期刊：
影响因子：
30.8
通讯作者：
Derbyshire,KeithM
中科院分区：
文献类型：
--
作者：
Wang,Jun;Parsons,LindaM;Derbyshire,KeithM
细菌偶联是一种主动过程，导致DNA从供体细胞向受体细胞单向转移。大多数转移系统是质粒编码的，需要蛋白质在一个独特的作用位点起作用来启动和完成DNA转移。相比之下，耻垢分枝杆菌的dna转移系统是染色体编码的。在这里，我们展示了染色体上存在的多重作用序列可以介导不可移动的测试质粒的转移。此外，与传统的质粒转移不同，受体重组功能需要允许该质粒及其衍生物通过类似于间隙修复的过程重新循环。为了促进修复，需要与受体染色体扩展DNA同源性，从而导致质粒获得受体染色体DNA。总之，这些结果表明，DNA转移在m。污垢发生的机制不同于典型的质粒转移系统。
Bacterial conjugation is an active process that results in unidirectional transfer of DNA from a donor to a recipient cell. Most transfer systems are plasmid-encoded and require proteins to act at a uniquecis-acting site to initiate and complete DNA transfer. By contrast, theMycobacterium smegmatisDNA transfer system is chromosomally encoded. Here we show that multiplecis-acting sequences present on the chromosome can mediate transfer of a non-mobilizable test plasmid. Moreover, unlike conventional plasmid transfer, recipient recombination functions are required to allow this plasmid, and derivatives of it, to re-circularize through a process similar to gap repair. Extended DNA homology with the recipient chromosome is required to facilitate repair, resulting in acquisition of recipient chromosomal DNA by the plasmid. Together, these results show that DNA transfer inM. smegmatisoccurs by a mechanism different from that of prototypical plasmid transfer systems.