Variability within the rabbit C repeats and sequences shared with other SINES.
Variability within the rabbit C repeats and sequences shared with other SINES.
复制标题
兔 C 重复序列内的变异性以及与其他 SINES 共享的序列。
DOI：
10.1093/nar/13.4.1073
复制
发表时间：
1985
影响因子：
14.9
通讯作者：
Printz,R
中科院分区：
文献类型：
--
作者：
Hardison,RC;Printz,R
短的散布重复序列（西内斯）的C家族在兔基因组中高度重复，并且大多数成员具有暗示通过重新插入RNA聚合酶III转录的RNA的双链拷贝来分散的模型的结构。我们已经确定了重复序列家族的其他成员的核苷酸序列，并将其编译以获得改进的共有序列。该编译显示，尽管重复序列的大多数区域是很保守的，但两个区域显示出高变异性。一些单独的重复序列被截短，并且一个截短的重复序列保留了逆转录子的特征结构。C重复序列的共有序列在大区域上与任何其他测序的哺乳动物SINE的序列不匹配，但观察到与几种灵长类动物和啮齿动物西内斯的短不完全匹配。与27个核苷酸的共有序列相似的序列 TCCCAGCAACCCACATGGGATGGCATGAGA 在所有被检查的哺乳动物西内斯中发现。该序列的3′部分与乳多空病毒复制起点处发现的DNA片段相匹配。
The C family of short, interspersed repeats (SINES) is highly repeated in the rabbit genome, and most members have a structure suggestive of a model for their dispersal via reinsertion of a double-stranded copy of an RNA polymerase III transcribed RNA. We have determined the nucleotide sequence of additional members of the repeat family and have compiled them to obtain an improved consensus sequence. This compilation shows that although most regions of the repeat are well conserved, two regions show high variability. Some individual repeats are truncated, and one truncated repeat retains the characteristic structures of a retroposon. The consensus sequence for C repeats does not match the sequence of any other sequenced mammalian SINE over large regions, but short imperfect matches to several primate and rodent SINES are observed. A sequence similar to the 27 nucleotide consensus sequence TCCCAGCAACCCACATGGGATGGCATGAGA was found in all mammalian SINES examined. The 3′ portion of this sequence matches a DNA segment found at the replication origins of papovaviruses.