Kinetic characterization of cytochrome c oxidase from Bacillus subtilis.
Kinetic characterization of cytochrome c oxidase from Bacillus subtilis.
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枯草芽孢杆菌细胞色素 C 氧化酶的动力学特征。
DOI：
10.1111/j.1432-1033.1987.tb13553.x
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
W. Konings
中科院分区：
文献类型：
--
作者：
W. Vrij;W. Konings
枯草芽孢杆菌aa3型细胞色素c氧化酶能够氧化不同来源的细胞色素c。酶的动力学性质受离子强度的影响。对酿酒酵母细胞色素c的亲和力随着离子强度的增加而下降，而Vmax几乎保持恒定。当将酶掺入人工膜中时，观察到Vmax增加。带负电荷的磷脂允许aa 3型氧化酶的高周转率。离子强度对马心细胞色素c氧化的影响导致Km和Vmax的显著变化。这些效应可以通过酶与底物相互作用的干扰来解释，而与酶聚集状态的变化无关。在人工膜中重构的酶的呼吸控制指数似乎依赖于磷脂组成、蛋白质/脂质比以及外部pH。离子载体尼日利亚菌素和缬氨霉素在不同pH值下对膜的酶活性和质子渗透性测量的作用表明，质子动力的两个分量，膜电位和pH梯度，原则上可以调节重组状态下的酶活性。
Bacillus subtilis aa3-type cytochrome c oxidase is capable of oxidizing cytochrome c from different origins. The kinetic properties of the enzyme are influenced by ionic strength. The affinity for Saccharomyces cerevisiae cytochrome c declines with increasing ionic strength whereas the Vmax remains almost constant. An increase of Vmax is observed when the enzyme is incorporated in artificial membranes. Negatively charged phospholipids allow high turnover rates of the aa3-type oxidase. The effect of ionic strength on oxidation of horse heart cytochrome c results in significant changes of both Km and Vmax. These effects can be explained by disturbances of enzyme-substrate interactions and are not related to changes in the aggregation state of the enzyme. The respiration control index of the enzyme reconstituted in artificial membranes appeared to be dependent on phospholipid composition, protein/lipid ratios and also on the external pH. The action of the ionophores nigericin and valinomycin, at various pH values, on the enzyme activity and proton-permeability measurements of the membranes indicate that both components of the proton-motive force, the membrane potential and the pH gradient, can in principle regulate enzyme activity in the reconstituted state.
DOI：
10.1016/s0021-9258(18)34936-6
发表时间：
1982-03
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
W E Jacobus;R W Moreadith;K M Vandegaer
通讯作者：
W E Jacobus;R W Moreadith;K M Vandegaer
球形红假单胞菌细胞色素 c 氧化酶的纯化和表征。
DOI：
10.1111/j.1432-1033.1982.tb06667.x
发表时间：
1982
期刊：
European journal of biochemistry
影响因子：
--
作者：
Gennis,RB;Casey,RP;Azzi,A;Ludwig,B
通讯作者：
Ludwig,B
嗜热栖热菌细胞色素 c1aa3 复合物的细胞色素 c 氧化酶活性研究。
DOI：
--
发表时间：
1984
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Yoshida,T;Fee,JA
通讯作者：
Fee,JA