Diagnosis of virus infection in orchid plants with high-resolution optical coherence tomography

Diagnosis of virus infection in orchid plants with high-resolution optical coherence tomography
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DOI:
10.1117/1.3066900
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发表时间:
2009-01-01
影响因子:
3.5
通讯作者:
Poh, Wee T.
Poh, Wee T.
中科院分区:
医学3区
文献类型:
--
作者:
Chow, Tzu H.;Tan, Khay M.;Poh, Wee T.

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这项工作研究了使用光学相干断层扫描(OCT)来识别兰花植物中的病毒感染。除了揭示兰花叶片的横截面结构,高散射上部叶表皮检测与OCT病毒感染的植物。在叶样品的组织学检查中观察不到这种明显的特征。此外,受胁迫但健康的植物的叶表皮表现出与病毒感染的植物相似的视觉症状,不是高度分散的,并且与健康植物的叶表皮相似。结果表明,病毒感染的兰花植物可以准确地识别通过成像的表皮层,他们的叶子与OCT。OCT模式是适合快速,无损诊断兰花病毒感染,这可能会导致显着的成本节约和更好地控制病毒的传播在兰花产业。(c)2009年,由光学仪器工程师协会(Society of Photo-Optical Instrumentation Engineers)主办。[DOI:10.1117/1.3066900]
This work investigates the use of optical coherence tomography (OCT) to identify virus infection in orchid plants. Besides revealing the cross-sectional structure of orchid leaves, highly scattering upper leaf epidermides are detected with OCT for virus-infected plants. This distinct feature is not observable under histological examination of the leaf samples. Furthermore, the leaf epidermides of stressed but healthy plants, which exhibit similar visual symptoms as virus-infected plants, are not highly scattering and are similar to those of healthy plants. The results suggest that virus-infected orchid plants can be accurately identified by imaging the epidermal layers of their leaves with OCT. The OCT modality is suitable for fast, nondestructive diagnosis of orchid virus infection, which may potentially lead to significant cost savings and better control of the spread of viruses in the orchid industry. (c) 2009 Society of Photo-Optical Instrumentation Engineers. [DOI: 10.1117/1.3066900]