Htd2p/Yhr067p is a yeast 3-hydroxyacyl-ACP dehydratase essential for mitochondrial function and morphology
Htd2p/Yhr067p is a yeast 3-hydroxyacyl-ACP dehydratase essential for mitochondrial function and morphology
复制标题
DOI：
10.1111/j.1365-2958.2004.04191.x
复制
发表时间：
2004-09-01
影响因子：
3.6
通讯作者：
Hiltunen, JK
中科院分区：
文献类型：
--
作者：
Kastaniotis, AJ;Autio, KJ;Hiltunen, JK
在酵母以及人类中，线粒体功能的最近认识到的方面之一是它们以丙二酰辅酶A依赖性方式合成脂肪酸的能力。我们在这里描述的3-羟酰基-ACP脱氢酶参与线粒体脂肪酸合成的鉴定。在酿酒酵母中应用菌落颜色分区筛选以寻找突变体，所述突变体当在不可发酵的碳源上生长时不能丢失携带编码肠道局部细菌类似物的嵌合构建体的质粒。我们的突变体，这是呼吸缺陷，缺乏细胞色素和显示异常的线粒体形态，被发现有一个病变的酵母YHR 067 w/RMD 12基因。Yhr 067 p被预测为硫酯酶/硫酯脱氢酶-异构酶超家族酶的成员。来自过表达YHR 067 w的细胞的线粒体提取物中的水合酶2活性增加。这些过表达的细胞还显示出显著的线粒体增大表型。我们得出结论，YHR 067 w编码一种新的线粒体3-（h），在bar下为羟基酰基-（t），在bar下为生物酯（d），在bar下为脱水酶（2），在bar下为，并建议将其重命名为HTD 2。无效和过表达突变体的线粒体表型表明YHR 067 w在维持酵母线粒体呼吸能力和形态中起关键作用。
Among the recently recognized aspects of mitochondrial functions, in yeast as well as humans, is their ability to synthesize fatty acids in a malonyl-CoA dependent manner. We describe here the identification of the 3-hydroxyacyl-ACP dehydratase involved in mitochondrial fatty acid synthesis. A colony-colour-sectoring screen was applied in Saccharomyces cerevisiae in a search for mutants that, when grown on a non-fermentable carbon source, were unable to lose a plasmid that carried a chimeric construct coding for mitochondrially localized bacterial analogue. Our mutants, which are respiratory deficient, lack cytochromes and display abnormal mitochondrial morphology, were found to have a lesion in the yeast YHR067w/RMD12 gene. The Yhr067p is predicted to be a member of the thioesterase/thioester dehydratase-isomerase superfamily enzymes. Hydratase 2 activity in mitochondrial extracts from cells overexpressing YHR067w was increased. These overexpressing cells also display a striking mitochondrial enlargement phenotype. We conclude that YHR067w encodes a novel mitochondrial 3-(h) under bar ydroxyacyl-(t) under bar hioester (d) under bar ehydratase (2) under bar and suggest renaming it HTD2. The mitochondrial phenotypes of the null and overexpression mutants suggest a crucial role of YHR067w in maintenance of mitochondrial respiratory competence and morphology in yeast.