Repair of an intercalated long bone defect with a synthetic biodegradable bone-inducing implant
Repair of an intercalated long bone defect with a synthetic biodegradable bone-inducing implant
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DOI：
10.1016/j.biomaterials.2005.01.054
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发表时间：
2005-09-01
期刊：
影响因子：
14
通讯作者：
Takaoka, K
中科院分区：
文献类型：
--
作者：
Yoneda, M;Terai, H;Takaoka, K
将重组人骨形态发生蛋白-2(rh BMP-2)与多孔的β-磷酸三钙(β-磷酸三钙)嵌段共聚，制备了一种新型的可完全吸收的成骨生物材料。在兔临界大小的骨缺损模型上，研究了重组人骨形态发生蛋白-2/聚乳酸-DX-聚乙二醇/β-磷酸三钙复合材料的骨再生能力。在最初的研究中，将聚乳酸-DX-聚乙二醇(250 Mg)和β-磷酸三钙(300 Mg)负载或不负载重组人骨形态发生蛋白2(50微克)的复合材料植入兔股骨1.5 cm的间质缺损处。术后8周前行双周X线片检查。只有在BNIP负荷组才能识别缺损区的骨性愈合。为了获得生物力学和重塑特性的进一步数据，另一组加载BNIP的复合材料被制作并观察长达24周。所有缺损均完全修复，无种植体残留痕迹。术后24周，组织学、三维CT(3D-CT)和力学测试显示修复骨的解剖和力学性能与未手术的股骨基本相当。这些实验结果表明，完全可吸收的重组人骨形成蛋白-2/聚乳酸-DX-聚乙二醇/β-磷酸三钙是一种有希望的复合材料，具有足够的成骨活性，可修复较大的骨缺损。(C)2005爱思唯尔有限公司。保留所有权利。
Recombinant human bone morphogenetic protein (rhBMP)-2 in a block copolymer composed of poly-D,L-lactic acid with randomly inserted p-dioxanone and polyethylene glycol (PLA-DX-PEG) as a carrier and porous beta-tricalcium phosphate (beta-TCP) blocks were used to generate a new fully absorbable osteogenic biomaterial. The bone regenerability of the rhBMP-2/PLA-DX-PEG/ beta-TCP composite was studied in a critical-sized rabbit bone defect model. In an initial study, a composite of PLA-DX-PEG (250mg) and beta-TCP (300 mg) loaded with or without rhBMP2 (50 mu g) was implanted into a 1.5 cm intercalated bone defect created in a rabbit femur. Defects were assessed by biweekly radiography until 8 weeks postoperatively. The bony union of the defect was recognized only in the BNIP-loaded group. To obtain further data on biomechanical and remodeling properties, another BNIP-loaded composites group was made and observed up to 24 weeks. All defects were completely repaired without residual traces of implants. Anatomical and mechanical properties of the repaired bone examined by histology, 3-dimensional CT (3D-CT) and mechanical testing were essentially equivalent to the nonoperated-on femur at 24 weeks. These experimental results indicate that fully absorbable rhBMP-2/PLA-DX-PEG/beta-TCP is a promising composite having osteogenicity efficient enough for repairing large bone defects. (c) 2005 Elsevier Ltd. All rights reserved.