Increased expression of (pro)renin receptor in the remnant kidneys of 5/6 nephrectomized rats
Increased expression of (pro)renin receptor in the remnant kidneys of 5/6 nephrectomized rats
复制标题
DOI：
10.1016/j.regpep.2009.11.006
复制
发表时间：
2010-01-08
影响因子：
--
通讯作者：
Imai, Yutaka
中科院分区：
文献类型：
--
作者：
Hirose, Takuo;Mori, Nobuyoshi;Imai, Yutaka
最近的研究表明，肾素（原）受体（（P）RR）是肾素-血管紧张素系统中新发现的成员，与肾器官损伤有关。然而，关于 (P)RR 表达在各种病理生理条件下的调节的信息很少。因此，我们通过定量 RT-PCR、Western blot 分析和免疫组织化学检查了 5/6 肾切除术后肾肿块消融大鼠残肾中 (P)RR 的表达。肾切除术后第56天，残肾中(P)RR mRNA表达水平较假手术组显着升高(约1.6倍，P=0.001)。 Western blot分析显示，第56天残肾中(P)RR蛋白表达量较假手术组显着升高(约7.9倍，P=0.02)。在5/6肾切除大鼠和假手术大鼠中，用抗(P)RR抗体对肾小管细胞进行免疫染色。 5/6 肾切除大鼠的肾小球偶尔有免疫染色，但假手术大鼠则没有。这些结果表明，5/6 肾切除大鼠的残余肾脏中肾内 (P)RR 表达增加，并表明 (P)RR 可能导致肾损伤。 (C) 2009 Elsevier B.V. 保留所有权利。
Recent studies have revealed that (pro)renin receptor ((P)RR), a newly identified member of the renin-angiotensin system, is associated with renal organ damage. However, there is little information regarding the regulation of (P)RR expression in various pathophysiological conditions. We therefore examined the expression of (P)RR in the remnant kidneys of rats with renal mass ablation due to 5/6 nephrectomy by quantitative RT-PCR, Western blot analysis and immunohistochemistry. Expression levels of (P)RR mRNA were significantly increased in the remnant kidneys at day 56 after nephrectomy, when compared with sham operation (about 1.6-fold, P=0.001). Western blot analysis showed that expression levels of (P)RR protein were greatly increased in the remnant kidneys at day 56, compared with sham operation (about 7.9-fold, P=0.02). The renal tubular cells were immunostained with anti-(P)RR antibody in both 5/6 nephrectomized rats and sham operated rats. The glomeruli were sporadically immunostained in 5/6 nephrectomized rats, but not in sham operated rats. These findings indicate that the intra-renal (P)RR expression is increased in the remnant kidneys of 5/6 nephrectomized rats, and suggest that (P)RR may contribute to the renal injury. (C) 2009 Elsevier B.V. All rights reserved.