Cheonggukjang Ethanol Extracts Inhibit a Murine Allergic Asthma via Suppression of Mast Cell-Dependent Anaphylactic Reactions
Cheonggukjang Ethanol Extracts Inhibit a Murine Allergic Asthma via Suppression of Mast Cell-Dependent Anaphylactic Reactions
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DOI：
10.1089/jmf.2013.2997
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发表时间：
2014-01-01
影响因子：
2.4
通讯作者：
Shon, Dong-Hwa
中科院分区：
文献类型：
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作者：
Bae, Min-Jung;Shin, Hee Soon;Shon, Dong-Hwa
清谷酱（CGJ）是韩国传统的发酵大豆食品，具有免疫调节作用。哮喘是最常见的慢性过敏性疾病，与环境过敏原的免疫反应有关。在哮喘的发病机制中，组胺是肥大细胞颗粒释放的重要炎症介质之一。在这项研究中，我们通过抑制组胺释放来评估 CGJ 对卵清蛋白 (OVA) 诱导的哮喘小鼠模型的治疗效果。 C57BL/6 小鼠通过腹腔注射 OVA 或磷酸盐缓冲盐水 (PBS) 对照致敏，然后通过吸入 OVA 进行攻击。用70％乙醇提取的CGJ(CGJE)(100mg/kg/天)或等效的PBS对小鼠进行腹膜内处理。通过支气管肺泡灌洗液细胞计数以及肺组织的组织病理学和免疫组织化学分析来评估哮喘相关炎症。为了阐明 CGJE 治疗抑制哮喘的机制，我们还检测了化合物 48/80 诱导的大鼠腹膜肥大细胞 (RPMC) 的脱颗粒和组胺释放。 CGJE治疗下调了OVA攻击小鼠肺部的嗜酸性粒细胞和单核细胞数量，并抑制了组织病理学变化，如嗜酸性粒细胞浸润、粘液积聚、杯状细胞增生和胶原纤维沉积。此外，CGJE 通过抑制钙 (Ca2+) 吸收以及炎症细胞浸润减轻耳肿胀，减轻了化合物 48/80 诱导的肥大细胞脱粒和 RPMC 组胺释放。这些发现表明 CGJE 可用作组胺介导性哮喘的抗哮喘膳食补充剂候选者。
Cheonggukjang(CGJ), a traditional Korean fermented soybean food, exerts immunomodulatory effects. Asthma is the most common chronic allergic disease to be associated with immune response to environmental allergens. In the pathogenesis of asthma, histamine is one of the important inflammatory mediators released from granules of mast cells. In this study, we evaluated the therapeutic effect of CGJ on a mouse model of ovalbumin (OVA)-induced asthma via the suppression of histamine release. C57BL/6 mice were sensitized by intraperitoneal injection of OVA or a phosphate-buffered saline (PBS) control and then challenged with OVA inhalation. Mice were treated intraperitoneally with either 70% ethanol-extracted CGJ (CGJE) (100 mg/kg/day) or equivalent PBS. Asthma-related inflammation was assessed by bronchoalveolar lavage fluid cell counts and histopathological and immunohistochemical analysis of lung tissues. To elucidate the mechanisms of asthma inhibition by CGJE treatment, we also examined degranulation and histamine release of compound 48/80-induced rat peritoneal mast cells (RPMCs). Treatment with CGJE downregulated the number of eosinophils and monocytes in the lungs of mice challenged with OVA and suppressed histopathological changes, such as eosinophil infiltration, mucus accumulation, goblet cell hyperplasia, and collagen fiber deposits. Moreover, CGJE alleviated compound 48/80-induced mast cell degranulation and histamine release from RPMCs through inhibition of calcium (Ca2+) uptake as well as ear swelling by infiltration of inflammatory cells. These findings demonstrated that CGJE can be used as an antiasthmatic dietary supplements candidate for histamine-mediated asthma.