Cutting Edge: Molecular Cloning of a Killer Cell Ig-Like Receptor in the Mouse and Rat 1
Cutting Edge: Molecular Cloning of a Killer Cell Ig-Like Receptor in the Mouse and Rat 1
复制标题
最前沿：小鼠和大鼠杀伤细胞 Ig 样受体的分子克隆 1
作者：
S. E. Hoelsbrekken;Øyvind Nylenna;P. C. Saether;I. Slettedal;J. Ryan;S. Fossum;E. Dissen
我们报告了小鼠和大鼠中KIR 3DL 1受体的分子克隆，其与灵长类杀伤细胞Ig样受体（KIRs/CD 158）具有37.4%至45.4%的相同性。小鼠和大鼠的分子都含有一对免疫受体酪氨酸为基础的抑制基序在其细胞质区域，表明抑制功能。Southern印迹分析表明，一个单一的KIR基因在大鼠，而小鼠基因组包含一个以上的KIR相关元件。大鼠Kir 3dl 1位点被定位于1号染色体上的白细胞受体基因复合体，而小鼠Kir 3dl 1定位于X染色体。RT-PCR结果表明，大鼠和小鼠NK细胞均选择性表达KIR 3DL 1。转染到293 T细胞中的小鼠KIR 3DL 1的表位标记的表达构建体诱导了55-kDa蛋白的表达。我们的数据表明，KIR受体可能有助于NK细胞受体库在啮齿动物，旁边的Ly-49家庭。
We report the molecular cloning of a KIR3DL1 receptor in the mouse and the rat, between 37.4 and 45.4% identical with primate killer cell Ig-like receptors (KIRs/CD158). Both mouse and rat molecules contain a pair of immunoreceptor tyrosine-based inhibition motifs in their cytoplasmic regions, suggesting an inhibitory function. Southern blot analysis indicated a single KIR gene in the rat, whereas the mouse genome contains more than one KIR-related element. The rat Kir3dl1 locus was mapped to the leukocyte receptor gene complex on chromosome 1, whereas mouse Kir3dl1 was localized to the X chromosome. RT-PCR demonstrated that KIR3DL1 was selectively expressed by NK cells in both rat and mouse. An epitope-tagged expression construct of mouse KIR3DL1 transfected into 293T cells induced expression of a ∼55-kDa protein. Our data indicate that KIR receptors may contribute to the NK cell receptor repertoire in rodents, alongside the Ly-49 family.