A universal code for RNA recognition by PUF proteins
A universal code for RNA recognition by PUF proteins
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DOI：
10.1038/nchembio.577
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发表时间：
2011-07-01
影响因子：
14.8
通讯作者：
Rackham, Oliver
中科院分区：
文献类型：
--
作者：
Filipovska, Aleksandra;Razif, Muhammad F. M.;Rackham, Oliver
可以结合任何感兴趣的RNA序列的蛋白质的设计具有许多潜在的生物学和医学应用。在这里，我们扩大了对Pumilio和FBF同源蛋白(PUF)重复序列的识别，超越了腺嘌呤、鸟嘌呤和尿嘧啶，并将它们进化为与胞嘧啶特异结合。这些重复序列可用于创建能够选择性结合不同序列和结构的RNA靶标的PUF结构域。
The design of proteins that can bind any RNA sequence of interest has many potential biological and medical applications. Here we have expanded the recognition of Pumilio and FBF homology protein (PUF) repeats beyond adenine, guanine and uracil and evolved them to specifically bind cytosine. These repeat sequences can be used to create PUF domains capable of selectively binding RNA targets of diverse sequence and structure.