Translocation of double-strand DNA through a silicon oxide nanopore
Translocation of double-strand DNA through a silicon oxide nanopore
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DOI：
10.1103/physreve.71.051903
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发表时间：
2005-05-01
影响因子：
2.4
通讯作者：
Dekker, C
中科院分区：
文献类型：
--
作者：
Storm, AJ;Chen, JH;Dekker, C
我们报告双链DNA易位实验使用氧化硅纳米孔直径约为10 nm。通过监测的电压偏置孔的电导，我们检测分子的长度范围从6557到48500碱基对。我们发现，分子可以通过孔在一个直线的方式和折叠状态。对环状DNA的实验进一步支持了这一观点。我们根据分子折叠状态对分子事件进行分类，并估计折叠位置。作为原理验证实验，我们表明纳米孔可用于区分混合物中存在的DNA片段的长度。这些实验为固态纳米孔的定量分析技术铺平了道路。
We report double-strand DNA translocation experiments using silicon oxide nanopores with a diameter of about 10 nm. By monitoring the conductance of a voltage-biased pore, we detect molecules with a length ranging from 6557 to 48 500 base pairs. We find that the molecules can pass the pore both in a straight linear fashion and in a folded state. Experiments on circular DNA further support this picture. We sort the molecular events according to their folding state and estimate the folding position. As a proof-of-principle experiment, we show that a nanopore can be used to distinguish the lengths of DNA fragments present in a mixture. These experiments pave the way for quantitative analytical techniques with solid-state nanopores.