Testing the feasibility of DNA typing for human identification by PCR and an oligonucleotide ligation assay.
Testing the feasibility of DNA typing for human identification by PCR and an oligonucleotide ligation assay.
复制标题
DOI：
--
复制
发表时间：
1996-06
影响因子：
9.8
通讯作者：
Claire Delahunty;Wendy Ankener;Qiang Deng;Jimmy Eng;Deborah A. Nickerson
中科院分区：
文献类型：
--
作者：
Claire Delahunty;Wendy Ankener;Qiang Deng;Jimmy Eng;Deborah A. Nickerson
DNA分型在人类基因组分析中的使用越来越多，并在法医和亲子鉴定领域得到广泛应用。在这份报告中，我们探讨了单核苷酸多态性（SNPs）分型的可行性，通过使用半自动化的方法分析人类DNA样本。在这种方法中，PCR用于扩增含有共同SNP的人类DNA片段。然后通过寡核苷酸连接测定（奥拉）的比色实施对扩增产物中的等位核苷酸进行分型。PCR/奥拉联合检测的结果直接由分光光度计读取;分析结果被编译;基因型被自动确定。已经开发了一组20个标记用于DNA分型，并使用来自CEPH家系（CEPH父母）的样本组进行了测试。这种分型的结果，以及将这种方法应用于更大的人群的潜力，进行了讨论。
The use of DNA typing in human genome analysis is increasing and finding widespread application in the area of forensic and paternity testing. In this report, we explore the feasibility of typing single nucleotide polymorphisms (SNPs) by using a semiautomated method for analyzing human DNA samples. In this approach, PCR is used to amplify segments of human DNA containing a common SNP. Allelic nucleotides in the amplified product are then typed by a colorimetric implementation of the oligonucleotide ligation assay (OLA). The results of the combined assay, PCR/OLA, are read directly by a spectrophotometer; the absorbances are compiled; and the genotypes are automatically determined. A panel of 20 markers has been developed for DNA typing and has been tested using a sample panel from the CEPH pedigrees (CEPH parents). The results of this typing, as well as the potential to apply this method to larger populations, are discussed.